Borinic Acid-Based Fluorogenic Probes as an Alternative to the Amplex Red Assay for Real-Time H2O2 Monitoring in Live Cells.

IF 3.5 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mathilde Pucher, Kirrthana Makenthirathasan, Hadrien Jalaber, Thomas LeSaux, Oliver Nüsse, Gilles Doisneau, Yann Bourdreux, Blaise Gatin-Fraudet, Ludovic Jullien, Boris Vauzeilles, Dominique Guianvarc'h, Marie Erard, Dominique Urban
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引用次数: 0

Abstract

Hydrogen peroxide (H2O2) is a crucial reactive oxygen species (ROS) involved in regulating both physiological and pathological processes. Excessive H2O2 production can lead to oxidative stress, contributing to aging, cancer, and neurodegenerative diseases. In contrast to other ROS exhibiting short lifespans, H2O2 is relatively stable, and its spatial and temporal dynamics are central to understanding its pathophysiological role. Therefore, the development of fluorescent probes that are highly selective, sensitive, and capable of a rapid response is still required. To date, numerous fluorescent probes have been developed. Among them, boronic acid triggers have attracted considerable attention but often suffer from limited reactivity, preventing real-time H2O2 monitoring. To overcome this lack of reactivity, we report the design and synthesis of new borinic acid-based fluorogenic probes for H2O2 detection in cellular environments. These probes are based on a hemicyanine scaffold functionalized with the borinic acid trigger, which demonstrated superior kinetics compared to its boronic counterpart. These probes enable efficient real-time monitoring of H2O2 in cellular models, both extracellularly and intracellularly. The kinetics of these enzyme-free chemical probes matched that of the gold standard Amplex UltraRed/horseradish peroxidase (HRP) assay, representing a significant advancement in the field and offering a versatile and sensitive tool for studying H2O2-mediated cell signaling.

基于硼酸的荧光探针作为一种替代Amplex Red法实时监测活细胞中H2O2的方法。
过氧化氢(H2O2)是一种重要的活性氧(ROS),参与调节生理和病理过程。过量的H2O2产生会导致氧化应激,导致衰老、癌症和神经退行性疾病。与其他活性氧寿命较短相比,H2O2相对稳定,其时空动态是理解其病理生理作用的核心。因此,仍然需要开发具有高选择性、高灵敏度和快速反应能力的荧光探针。迄今为止,已经开发了许多荧光探针。其中,硼酸触发器备受关注,但往往反应性有限,无法实时监测H2O2。为了克服这种缺乏反应性,我们报道了设计和合成新的基于硼酸的荧光探针,用于细胞环境中H2O2的检测。这些探针是基于半花青碱支架功能化的硼酸触发器,与硼的对应物相比,表现出优越的动力学。这些探针能够有效地实时监测细胞模型中的H2O2,包括细胞外和细胞内。这些无酶化学探针的动力学与金标准Amplex红外/辣根过氧化物酶(HRP)测定相匹配,代表了该领域的重大进步,并为研究h2o2介导的细胞信号传导提供了一个多功能和敏感的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Chemical Biology
ACS Chemical Biology 生物-生化与分子生物学
CiteScore
7.50
自引率
5.00%
发文量
353
审稿时长
3.3 months
期刊介绍: ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology. The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies. We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.
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