Fermented bile improved growth performance and intestine health by altering metabolic profiles and intestinal microbes in Chinese perch, Siniperca chuatsi

IF 2.3 3区 农林科学 Q2 FISHERIES
Qing Guo, Yutao Liu, Lu Zhao, Wenwen Liu, Zhihang Zhu, Houfa Zhao, Cuimin Mu, Xuepeng Wang
{"title":"Fermented bile improved growth performance and intestine health by altering metabolic profiles and intestinal microbes in Chinese perch, Siniperca chuatsi","authors":"Qing Guo,&nbsp;Yutao Liu,&nbsp;Lu Zhao,&nbsp;Wenwen Liu,&nbsp;Zhihang Zhu,&nbsp;Houfa Zhao,&nbsp;Cuimin Mu,&nbsp;Xuepeng Wang","doi":"10.1111/jwas.70034","DOIUrl":null,"url":null,"abstract":"<p>A type of fermented bile acid (BA) named 170HDa was produced using a biological method, and its impacts on growth performance, metabolism, and intestinal microbes in Chinese perch (<i>Siniperca chuatsi</i>) were investigated. Seven experimental diets were established, including treatment groups supplemented with 0.02%–0.06% 170HDa (GN1-GN5), a positive control diet containing 0.04% commercial BAs (GN6), and a negative control diet without BA supplementation (GN7). The trial lasted for 6 weeks. Results demonstrated significant improvements (<i>p</i> &lt; 0.05) in growth indicators such as final weight (FW), weight gain (WG), specific growth rate (SGR), and feed conversion ratio (FCR) in fish fed diets containing 0.02%–0.04% 170HDa compared to the control groups. Serum biochemical analyses revealed that dietary 170HDa improved lipid metabolism by significantly reducing low-density lipoprotein cholesterol (LDL-C) and amylase (AMS) (<i>p</i> &lt; 0.05). Histopathological examination indicated that 0.02%–0.04% 170HDa-treated fish showed more intact intestinal villi and less inflammatory damage, whereas the control group displayed notable intestinal damage characterized by inflammatory cell infiltration, lysis, and severe vacuolization. Gut microbial diversity was significantly affected by 170HDa treatment, particularly at the phylum (Proteobacteria, Tenericutes, Firmicutes, and Fusobacteria) and genus levels. Moreover, the highest activities of acid and alkaline phosphatase appeared in the GN3 group. Meanwhile, elevated levels of IL-1β, TNFα and NF-kB p65 were found in the 170HDa-treated groups. These findings suggest that dietary 170HDa at a concentration of 0.02%–0.04% effectively enhanced growth performance, lipid metabolism, and intestine health in Chinese perch, potentially offering an environmentally friendly alternative to chemically extracted BAs.</p>","PeriodicalId":17284,"journal":{"name":"Journal of The World Aquaculture Society","volume":"56 4","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jwas.70034","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The World Aquaculture Society","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jwas.70034","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

Abstract

A type of fermented bile acid (BA) named 170HDa was produced using a biological method, and its impacts on growth performance, metabolism, and intestinal microbes in Chinese perch (Siniperca chuatsi) were investigated. Seven experimental diets were established, including treatment groups supplemented with 0.02%–0.06% 170HDa (GN1-GN5), a positive control diet containing 0.04% commercial BAs (GN6), and a negative control diet without BA supplementation (GN7). The trial lasted for 6 weeks. Results demonstrated significant improvements (p < 0.05) in growth indicators such as final weight (FW), weight gain (WG), specific growth rate (SGR), and feed conversion ratio (FCR) in fish fed diets containing 0.02%–0.04% 170HDa compared to the control groups. Serum biochemical analyses revealed that dietary 170HDa improved lipid metabolism by significantly reducing low-density lipoprotein cholesterol (LDL-C) and amylase (AMS) (p < 0.05). Histopathological examination indicated that 0.02%–0.04% 170HDa-treated fish showed more intact intestinal villi and less inflammatory damage, whereas the control group displayed notable intestinal damage characterized by inflammatory cell infiltration, lysis, and severe vacuolization. Gut microbial diversity was significantly affected by 170HDa treatment, particularly at the phylum (Proteobacteria, Tenericutes, Firmicutes, and Fusobacteria) and genus levels. Moreover, the highest activities of acid and alkaline phosphatase appeared in the GN3 group. Meanwhile, elevated levels of IL-1β, TNFα and NF-kB p65 were found in the 170HDa-treated groups. These findings suggest that dietary 170HDa at a concentration of 0.02%–0.04% effectively enhanced growth performance, lipid metabolism, and intestine health in Chinese perch, potentially offering an environmentally friendly alternative to chemically extracted BAs.

Abstract Image

发酵胆汁通过改变中华鲈鱼(sininiperca chuatsi)的代谢谱和肠道微生物来改善其生长性能和肠道健康
采用生物法制备一种发酵胆汁酸(BA) 170HDa,研究其对chuatsi中华鲈鱼(sininiperca chuatsi)生长性能、代谢和肠道微生物的影响。共设置7种试验饲粮,包括添加0.02% ~ 0.06% 170HDa的试验组(GN1-GN5)、添加0.04%商业BAs的阳性对照饲粮(GN6)和不添加BA的阴性对照饲粮(GN7)。试验期6周。结果表明,与对照组相比,添加0.02% ~ 0.04% 170HDa的饲料对鱼的末重(FW)、增重(WG)、特定生长率(SGR)和饲料系数(FCR)等生长指标均有显著改善(p < 0.05)。血清生化分析显示,饲粮170HDa通过显著降低低密度脂蛋白胆固醇(LDL-C)和淀粉酶(AMS)改善脂质代谢(p < 0.05)。组织病理学检查显示,0.02%-0.04% 170hda处理的鱼肠绒毛更完整,炎症损伤更小,而对照组肠损伤明显,炎症细胞浸润、溶解、严重空泡化。170HDa处理显著影响了肠道微生物多样性,特别是在门(变形菌门、微内门、厚壁菌门和梭杆菌门)和属水平上。酸性和碱性磷酸酶活性均以GN3组最高。同时,在170hda处理组中发现IL-1β、TNFα和NF-kB p65水平升高。综上所述,饲粮中添加0.02% ~ 0.04%浓度的170HDa可有效提高鲈鱼的生长性能、脂质代谢和肠道健康,有望成为化学提取ba的环保替代品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.90
自引率
7.10%
发文量
69
审稿时长
2 months
期刊介绍: The Journal of the World Aquaculture Society is an international scientific journal publishing original research on the culture of aquatic plants and animals including: Nutrition; Disease; Genetics and breeding; Physiology; Environmental quality; Culture systems engineering; Husbandry practices; Economics and marketing.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信