Detection and Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Raw Seafood From the Coastal Area of Bangladesh

IF 3.9 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2025-06-27 DOI:10.1002/mbo3.70023

Zannatul Firdous, Md. Saiful Islam, Md. Ashek Ullah, Md. Liton Rana, Farhana Binte Ferdous, Al-Muksit Mohammad Taufiquer Rahman, Jayedul Hassan, Md. Tanvir Rahman

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Abstract

The emergence of antimicrobial resistance (AMR) and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) in seafood represents a significant public health concern. In this study, we screened 102 raw seafood samples, comprising shrimp (n = 42), sea fish (n = 36), and crabs (n = 24), to detect ESBL-EC. E. coli was isolated and identified through culture-based methods, staining procedures, biochemical assays, and polymerase chain reaction (PCR) analysis. The AMR properties of E. coli isolates were evaluated using the disc diffusion test, while ESBL-EC was identified phenotypically through the double-disc synergy test and confirmed at the genetic level using PCR. PCR analysis revealed that 42.2% (43/102) of the samples were contaminated with E. coli, with sea fish showing the highest (p < 0.05) prevalence (63.9%, 23/36), followed by crabs (37.5%, 9/24) and shrimp (26.2%, 11/42). All the isolates exhibited phenotypic resistance to ampicillin, followed by ceftazidime (95.3%), ciprofloxacin (55.8%), azithromycin (39.5%), cefotaxime (37.2%), and streptomycin (16.3%). Notably, 69.8% (30/43) of E. coli isolates exhibited phenotypically multidrug resistance. Moreover, 18.6% (8/43) of the isolates showed ESBL-producing characteristics, higher in shrimp than in sea fish and crabs. ESBL-related gene, bla_TEM, was detected in 75% (6/8), bla_SHV in 87.5% (7/8), and bla_CTX-M in 50% (4/8) of the ESBL-EC isolates. Regular surveillance of seafood for antimicrobial-resistant bacteria, particularly ESBL-producing strains, is recommended due to their potential public health implications.

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孟加拉沿海生海产产β -内酰胺酶大肠杆菌的广谱检测与鉴定

海产品中抗菌素耐药性（AMR）和产生广谱β -内酰胺酶（ESBL）的大肠杆菌（ESBL- ec）的出现是一个重大的公共卫生问题。在本研究中，我们筛选了102份生海鲜样品，包括虾（n = 42）、海鱼（n = 36）和螃蟹（n = 24），检测ESBL-EC。通过培养方法、染色程序、生化试验和聚合酶链反应（PCR）分析分离和鉴定大肠杆菌。采用圆盘扩散试验对大肠杆菌分离株的AMR特性进行了评价，采用双圆盘协同试验对ESBL-EC进行了表型鉴定，并采用PCR在遗传水平上进行了证实。PCR检测结果显示，42.2%（43/102）的样品中大肠杆菌感染率最高（p < 0.05），其中海鱼感染率最高（63.9%,23/36），螃蟹次之（37.5%,9/24），虾类次之（26.2%,11/42）。所有菌株对氨苄西林均表现出表型耐药，其次是头孢他啶（95.3%）、环丙沙星（55.8%）、阿奇霉素（39.5%）、头孢噻肟（37.2%）和链霉素（16.3%）。值得注意的是，69.8%（30/43）的大肠杆菌分离株表现出表型多药耐药。18.6%（8/43）的分离株具有产esbl的特征，虾类高于海鱼和蟹类。在ESBL-EC分离株中，有75%（6/8）检测到esbl相关基因blaTEM， 87.5%（7/8）检测到blaSHV， 50%（4/8）检测到blaCTX-M。建议定期监测海产品中的抗微生物细菌，特别是产生esbl的菌株，因为它们可能对公共卫生产生影响。

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

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