Biomimetic synthesis of TiO2 nanoparticles by Sphingomonas sp. and their cytotoxicity assessment on Allium cepa root tips

Abstract

The current research identifies the bacterial synthesis of titanium oxide nanoparticles from Sphingomonas sp. towards cytotoxicity of Allium cepa root tips and its biological activity. Green synthesized TiO₂ nanoparticles are eco-friendly as they don’t release any harmful chemicals into the environment and are less cost when compared to chemically synthesized nanoparticles. The biomimetic TiO₂ nanoparticles are used in a friendly manner in Bioremediation, Cosmetics, Painting, Electricals and Medicine, etc,. The Sphingomonas sp. Synthesised TiO₂ nanoparticles proved the characterization study of UV visible spectrophotometry at 348 nm and with the exhibiting band gap energy of 3.1 eV. The Fourier Transform Infrared (FTIR) spectroscopy has the strong peak of TiO₂ nanoparticles at 1141 cm⁻¹, and XRD has a grain size of 37.55 nm. The shape of SEM image confirms that it was a TiO₂ nanoparticles, which was in spherical shape whereas the TEM image was identified as a cubic image. In this research we also evaluated the antioxidant activity, anti-inflammatory, biofilm inhibition and in antimicrobial activity Pseudomonas Sp. shows the maximum resistant of TiO₂ nanoparticles synthesized from Sphingomonas sp. The cytotoxicity of Allium cepa root tips treated with TiO₂ nanoparticles exhibited less toxicity, but there were slight changes in chromosomes which enhanced the plant growth.