Dietary vitamin D3 improves growth performance and hepatic glycolipid metabolism in largemouth bass (Micropterus salmoides)

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-06-23 DOI:10.1016/j.anifeedsci.2025.116429

Ju Zhao , Quanquan Cao , Mingyao Yan , Yonglan Ye , Caterina Faggio , Haifeng Liu , Jun Jiang

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引用次数: 0

Abstract

Vitamin D3 (VD₃) has been recognized to improve glycolipid metabolism, whereas the mechanism behind is still unknown. Herein, largemouth bass, Micropterus salmoides (LMB) were fed with six diets with different concentrations of VD₃ for 12-weeks. The results demonstrated that VD₃ improved growth, feed utilization and proximate composition. In addition, VD₃ enhanced liver glycogen content, glycolysis-related genes, glycogen synthase kinase 3β a (GSK3β a) and insulin receptor a (INSRa) mRNA expression, suppressed plasma glucose content and gluconeogenesis-related genes expression. VD₃ down-regulated forkhead box O1a (FoxO1a) mRNA expression and nucleus-FoxO1 protein level and increased cytoplasm-FoxO1 protein level. VD₃ decreased plasma triglyceride (TG), total cholesterol (TC) contents and aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities. Histological analyses revealed that VD₃ decreased macrovesicular steatosis of liver. VD₃ improved liver lipid metabolism via spursing lipolysis, transportation, and inhibiting lipogenesis. RT-PCR and western blot tests suggested that the potential mechanism may partially be attributed to the VDR/AMPK/SIRT1 pathway mediated glycolipid metabolism. According to the broken line regression analysis of PWG, the dietary VD₃ requirement of LMB was estimated to be 1980.86 IU/kg. This study provides theoretical support and a novel mechanism of action VD₃ glycolipid metabolism via VDR/AMPK/SIRT1 pathway.

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饲料中维生素D3改善大口黑鲈生长性能和肝脏糖脂代谢

维生素D3 （VD3）已被认为可以改善糖脂代谢，但其背后的机制尚不清楚。本试验采用6种添加不同浓度VD3的饲料饲喂大口黑鲈（Micropterus salmoides） 12周。结果表明，VD3提高了生长、饲料利用率和近似组成。此外，VD3可提高肝糖原含量、糖酵解相关基因、糖原合成酶激酶3β a （GSK3β a）和胰岛素受体a (INSRa) mRNA表达，抑制血浆葡萄糖含量和糖异生相关基因表达。VD3下调叉头盒O1a (FoxO1a) mRNA表达和细胞核- foxo1蛋白水平，上调细胞质- foxo1蛋白水平。VD3降低血浆甘油三酯（TG）、总胆固醇（TC）含量和天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）活性。组织学分析显示，VD3可减少肝脏大泡性脂肪变性。VD3通过刺激脂肪分解、运输和抑制脂肪生成来改善肝脏脂质代谢。RT-PCR和western blot检测提示，其潜在机制可能部分归因于VDR/AMPK/SIRT1通路介导的糖脂代谢。根据PWG折线回归分析，估计LMB日粮VD3需取量为1980.86 IU/kg。本研究为VD3糖脂代谢通过VDR/AMPK/SIRT1通路的作用提供了理论支持和新机制。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.