Diet-derived LPS determines intestinal IgA induction and repertoire characteristics independently of the microbiota

IF 25.5 1区 医学 Q1 IMMUNOLOGY
Catherine Mooser, Francesca Ronchi, Julien P. Limenitakis, Cristina Kalbermatter, Sandro Christensen, Mercedes Gomez de Agüero, Tobias Fuhrer, Uwe Sauer, Andrew J. Macpherson, Stephanie C. Ganal-Vonarburg
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引用次数: 0

Abstract

A hallmark of the main secreted antibody immunoglobulin A (IgA) is its mutational load that accumulates throughout life. Although this is mainly interpreted in terms of continuing microbial induction, we show that dietary composition during early life can promote IgA induction, its repertoire, and mutational diversification independently of microbial exposure. Using germ-free and colonized mice fed different diets formulated with proprietary grain-based processing or from purified chemicals with different principal macronutrient calorie sources, we found that dietary lipopolysaccharide contamination led to Toll-like receptor (TLR) 4 signaling and promoted germinal center activity in the intestinal immune compartment. The effects of lipopolysaccharide on mucosal immune induction were phenocopied only when presented within colloidal liposomes rather than in dispersed solution. These findings indicate that dietary composition and its formulation can leave a durable impression on the resultant IgA repertoire.
饮食来源的LPS决定了肠道IgA诱导和菌群特征,而不依赖于微生物群
主要分泌抗体免疫球蛋白A (IgA)的一个标志是其在整个生命中积累的突变负荷。尽管这主要是根据持续的微生物诱导来解释的,但我们表明,生命早期的饮食组成可以促进IgA诱导,其曲目和突变多样化,而不依赖于微生物暴露。研究人员使用无菌小鼠和定植小鼠饲喂不同的饲粮,这些饲粮由专有的谷物加工配制,或由含有不同主要巨量营养素卡路里来源的纯化化学品配制,结果发现,饲粮中的脂多糖污染导致toll样受体(TLR) 4信号传导,并促进肠道免疫区生发中心的活性。脂多糖对粘膜免疫诱导的影响仅在胶体脂质体中呈现,而不是在分散溶液中呈现。这些发现表明,饲料成分及其配方可以对最终产生的IgA库留下持久的印象。
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来源期刊
Immunity
Immunity 医学-免疫学
CiteScore
49.40
自引率
2.20%
发文量
205
审稿时长
6 months
期刊介绍: Immunity is a publication that focuses on publishing significant advancements in research related to immunology. We encourage the submission of studies that offer groundbreaking immunological discoveries, whether at the molecular, cellular, or whole organism level. Topics of interest encompass a wide range, such as cancer, infectious diseases, neuroimmunology, autoimmune diseases, allergies, mucosal immunity, metabolic diseases, and homeostasis.
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