Influence of Aging and Diabetes on the Mechanical Properties of Mouse Skin.

IF 1.6 Q3 DERMATOLOGY
Sarah Miny, Gaël Runel, Julien Chlasta, Christelle Bonod
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Abstract

Background: Diabetics accumulate Advanced Glycation End products (AGEs) such as Nε-(carboxymethyl)lysine (CML) in their skin, which can provoke changes in the skin's biomechanical properties. The same changes are also observed during aging. Collagen is one of the first targets of glycation, and this leads to the disruption of the dermis, potentially contributing to the skin complications seen in diabetes, like impaired wound healing and the formation of chronic ulcers. We therefore investigated whether it was possible to detect differences in the biomechanical properties of the reticular dermis by comparing C57/BL6 control mice, type 1 and type 2 diabetic mice, and aged mice.

Methods: To investigate this, we used an Atomic Force Microscope (a type of local probe microscope used to visualize the surface topography of a sample) to measure the elastic modulus of each skin sample. The elastic modulus is a parameter that describes a tissue's resistance to elastic deformation when stress is applied. We also determined whether diabetes is associated with the accumulation of AGEs via Western blots.

Results: We found that type 2 diabetic mice and aged mice had a stiffer reticular dermis than young control mice. No differences were found in type 1 diabetic mice. The results of the Western blot did not reveal any significant differences in the CML content in different types of mice, although a non-significant increase was found in type 2 diabetic and aged mice. We show that there is a significant positive correlation between the amount of CML in a mouse and the rigidity of its reticular dermis.

Conclusions/interpretation: We have demonstrated that increased glycation in mouse skin is correlated with the biomechanical properties of that skin, which explains the wound healing defects diabetic patient's experience. AFM is therefore a powerful technique that could be used to characterize the mechanical effects of treatments aimed at reducing the level of AGEs in the skin.

衰老和糖尿病对小鼠皮肤力学性能的影响。
背景:糖尿病患者皮肤中积累了晚期糖基化终产物(AGEs),如Nε-(羧甲基)赖氨酸(CML),这会引起皮肤生物力学特性的变化。在老化过程中也观察到同样的变化。胶原蛋白是糖基化的首要目标之一,这会导致真皮层的破坏,可能导致糖尿病中出现的皮肤并发症,如伤口愈合受损和慢性溃疡的形成。因此,我们研究了是否有可能通过比较C57/BL6对照小鼠、1型和2型糖尿病小鼠以及老年小鼠来检测网状真皮层生物力学特性的差异。方法:为了研究这一点,我们使用原子力显微镜(一种局部探针显微镜,用于可视化样品的表面形貌)来测量每个皮肤样品的弹性模量。弹性模量是描述组织在施加应力时抵抗弹性变形的参数。我们还通过Western blots确定糖尿病是否与AGEs积累有关。结果:我们发现2型糖尿病小鼠和老年小鼠的网状真皮比年轻对照小鼠更硬。在1型糖尿病小鼠中没有发现差异。Western blot结果显示不同类型小鼠CML含量无显著差异,但2型糖尿病小鼠和老年小鼠CML含量无显著增加。我们发现,在小鼠中CML的数量与其网状真皮的刚性之间存在显著的正相关。结论/解释:我们已经证明,小鼠皮肤中糖基化的增加与皮肤的生物力学特性相关,这解释了糖尿病患者伤口愈合缺陷的经历。因此,AFM是一种强大的技术,可用于表征旨在降低皮肤中AGEs水平的治疗的机械效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Dermatopathology
Dermatopathology DERMATOLOGY-
自引率
5.30%
发文量
39
审稿时长
11 weeks
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