Bioassay Using the DR-EcoScreen System to Measure Dioxin-Related Compounds in Serum Samples from Individuals Exposed to Dioxins Originating from Agent Orange in Vietnam.

IF 3.9 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Toxics Pub Date : 2025-06-19 DOI:10.3390/toxics13060513
Masafumi Nakamura, Ryo Matsuda, Hoa Thi Vu, Tai Pham-The, Thao Ngoc Pham, Tomoya Takiguchi, Hisao Nishijo, Muneko Nishijo
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Abstract

A bioassay for dioxin analysis of human samples has the advantages of cost effectiveness and requiring only a small sample volume. Using a DR-EcoScreen bioassay, we measured the biological equivalency (BEQ) levels in serum samples from 32 men exposed to dioxins in Bien Hoa and 32 unexposed men in Hanoi, Vietnam. For the Bien Hoa men, the World Health Organization toxic equivalent (WHO-TEQ) levels of dioxins had already been measured by instrumental analysis. The difference in fat-based BEQ levels between exposed and unexposed men was greater than for crude BEQ levels, with a strong correlation between fat-based BEQ and WHO-TEQ levels. The fat-based BEQ levels in Bien Hoa men with longer residency but lower WHO-TEQ levels were significantly higher than those in unexposed men and Bien Hoa men with shorter residency but higher WHO-TEQs, suggesting that fat-based BEQ may be an effective marker of dioxin-like activity. Additionally, comparisons of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and TEQs between shorter- and longer-residency groups indicated that higher levels of polychlorinated dibenzo-p-dioxins (PCDDs), particularly TCDD, contribute to increased BEQ levels. Taken together, the DR-EcoScreen bioassay may be useful to analyze dioxin-like activity associated with WHO-TEQs of men in a dioxin contamination hotspot originating from Agent Orange in Vietnam.

使用DR-EcoScreen系统测定越南接触源自橙剂的二恶英个体血清样本中二恶英相关化合物的生物测定
对人类样本进行二恶英分析的生物测定具有成本效益和只需要小样本量的优点。使用DR-EcoScreen生物测定法,我们测量了边化32名暴露于二恶英的男性和越南河内32名未暴露于二恶英的男性的血清样本中的生物等效性(BEQ)水平。对于边和男子,已经通过仪器分析测量了世界卫生组织的毒性当量(WHO-TEQ)二恶英水平。暴露者与未暴露者之间脂肪基BEQ水平的差异大于粗BEQ水平,脂肪基BEQ与WHO-TEQ水平之间存在很强的相关性。居住时间较长但WHO-TEQ水平较低的边化男性的脂肪基BEQ水平显著高于未暴露的男性和居住时间较短但WHO-TEQ水平较高的边化男性,这表明脂肪基BEQ可能是二恶英样活性的有效标志。此外,对2,3,7,8-四氯二苯并-对二恶英(TCDD)和teq在居住时间较短和较长两组之间的比较表明,较高水平的多氯二苯并-对二恶英(pcdd),特别是TCDD,导致了BEQ水平的升高。总之,DR-EcoScreen生物测定法可能有助于分析越南橙剂二恶英污染热点地区男性与who - teq相关的二恶英样活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Toxics
Toxics Chemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍: Toxics (ISSN 2305-6304) is an international, peer-reviewed, open access journal which provides an advanced forum for studies related to all aspects of toxic chemicals and materials. It publishes reviews, regular research papers, and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in detail. There is, therefore, no restriction on the maximum length of the papers, although authors should write their papers in a clear and concise way. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of calculations and experimental procedure can be deposited as supplementary material, if it is not possible to publish them along with the text.
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