{"title":"Electrophoretic protein profiles in seminal plasma and sperm: a comparative study of Bali and Wagyu Bulls.","authors":"Tulus Maulana, Panca Andes Hendrawan, Sutikno Sutikno, Pajri Anwar, Mokhamad Fakhrul Ulum, Rudy Priyanto, Jakaria Jakaria","doi":"10.5455/OVJ.2025.v15.i5.18","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Seminal plasma and sperm play vital roles in fertility because they contain various proteins that influence sperm quality and function.</p><p><strong>Aim: </strong>This study aimed to compare the protein profiles of seminal plasma and sperm in Bali and Wagyu cattle and evaluate their correlation with fresh semen quality.</p><p><strong>Methods: </strong>The study was conducted at the Singosari National Artificial Insemination Center using 3 Bali and 3 Wagyu superior bulls aged 5-12 years. Sperm motility parameters were analyzed using computer-assisted sperm analysis (IVOS-Hamilton). Sperm were extracted using the PRO-PREPTM Protein Extraction Solution. Sperm and seminal plasma protein concentrations were measured using the BCA protein assay, and 1D SDS-PAGE was used to analyze protein profiles. The gels were stained with Coomassie Brilliant Blue to visualize protein bands.</p><p><strong>Results: </strong>Comparative analysis of semen characteristics, motility parameters, and kinematic parameters between Wagyu and Bali bulls revealed no significant differences. The seminal plasma protein concentrations in Bali bulls and Wagyu did not show a statistically significant difference. The sperm protein concentration in Bali bulls was significantly higher compared to Wagyu. Seminal plasma analysis of Bali bulls showed a diverse protein profile and Wagyu semen plasma had fewer bands, reflecting breed-specific differences. In contrast, sperm analysis of Bali bulls and Wagyu consistently showed heavy protein bands on sperm function and viability.</p><p><strong>Conclusion: </strong>Bali bulls showed greater variation in spermatozoa protein than Wagyu, with protein differences at molecular weights of 60-63 kDa, 35 kDa, and 18-20 kDa. In addition, a positive correlation was found between the number of motile spermatozoa and the number of straws per ejaculate.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"15 5","pages":"2021-2029"},"PeriodicalIF":1.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184453/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2025.v15.i5.18","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Seminal plasma and sperm play vital roles in fertility because they contain various proteins that influence sperm quality and function.
Aim: This study aimed to compare the protein profiles of seminal plasma and sperm in Bali and Wagyu cattle and evaluate their correlation with fresh semen quality.
Methods: The study was conducted at the Singosari National Artificial Insemination Center using 3 Bali and 3 Wagyu superior bulls aged 5-12 years. Sperm motility parameters were analyzed using computer-assisted sperm analysis (IVOS-Hamilton). Sperm were extracted using the PRO-PREPTM Protein Extraction Solution. Sperm and seminal plasma protein concentrations were measured using the BCA protein assay, and 1D SDS-PAGE was used to analyze protein profiles. The gels were stained with Coomassie Brilliant Blue to visualize protein bands.
Results: Comparative analysis of semen characteristics, motility parameters, and kinematic parameters between Wagyu and Bali bulls revealed no significant differences. The seminal plasma protein concentrations in Bali bulls and Wagyu did not show a statistically significant difference. The sperm protein concentration in Bali bulls was significantly higher compared to Wagyu. Seminal plasma analysis of Bali bulls showed a diverse protein profile and Wagyu semen plasma had fewer bands, reflecting breed-specific differences. In contrast, sperm analysis of Bali bulls and Wagyu consistently showed heavy protein bands on sperm function and viability.
Conclusion: Bali bulls showed greater variation in spermatozoa protein than Wagyu, with protein differences at molecular weights of 60-63 kDa, 35 kDa, and 18-20 kDa. In addition, a positive correlation was found between the number of motile spermatozoa and the number of straws per ejaculate.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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