Metabolomic Profiling Reveals PGPR-Driven Drought Tolerance in Contrasting Brassica juncea Genotypes.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metabolites Pub Date : 2025-06-19 DOI:10.3390/metabo15060416
Asha Rani Sheoran, Nita Lakra, Baljeet Singh Saharan, Annu Luhach, Yogesh K Ahlawat, Rosa Porcel, Jose M Mulet, Prabhakar Singh
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引用次数: 0

Abstract

Background: Drought stress is a major abiotic factor limiting Brassica juncea productivity, resulting in significant yield reductions. Plant Growth-Promoting Rhizobacteria (PGPR) have shown potential in enhancing drought tolerance; however, the metabolomic changes associated with their effects remain largely unexplored. This study examines the metabolic changes induced by a PGPR consortium (Enterobacter hormaechei, Pantoea dispersa, and Acinetobacter sp.) in two contrasting genotypes B. juncea (L.) Czern. 'RH 725' (drought tolerant) and B. juncea (L.) Czern. 'RH-749' (drought sensitive for drought tolerance, under both control and drought conditions.

Methods: Metabolite profiling was conducted using gas chromatography-mass spectrometry (GC-MS) to identify compounds that accumulated differentially across treatments. We applied multivariate statistical methods, such as Partial Least Squares Discriminant Analysis (PLS-DA), hierarchical clustering, and pathway enrichment analysis, to explore metabolic reprogramming.

Results: Drought stress induced significant changes in metabolite profile, particularly increasing the levels of osmoprotectants such as trehalose, glucose, sucrose, proline, and valine. Additionally, alterations in organic acids (malic acid and citric acid) and fatty acids (oleic acid and linoleic acid) were observed. PGPR inoculation further amplified these metabolic responses to enhance the osmotic regulation, reactive oxygen species (ROS) detoxification, and carbon-nitrogen metabolism, with RH-725 displaying a stronger adaptive response. Pathway enrichment analysis revealed that PGPR treatment significantly influenced metabolic pathways related to starch and sucrose metabolism, galactose metabolism, and amino acid biosynthesis, which play critical roles in drought adaptation.

Conclusion: These findings provide insights into how PGPR contributes to stress resilience in B. juncea by modulating key biochemical pathways. This study provides new molecular insights into the known effect of PGPR for mitigating drought stress in oilseed crops.

代谢组学分析揭示不同芥菜基因型中pgpr驱动的耐旱性
背景:干旱胁迫是限制芥菜产量的主要非生物因素,导致其产量显著下降。植物促生长根瘤菌(Plant growth promoting Rhizobacteria, PGPR)具有增强抗旱性的潜力;然而,与它们的影响相关的代谢组学变化在很大程度上仍未被探索。本研究探讨了一个PGPR联盟(hormaecheenterobacter, Pantoea分散菌和Acinetobacter sp.)在两种不同基因型juncea (L.)中诱导的代谢变化。Czern。‘RH 725’(耐旱)和B. juncea (L.)Czern。RH-749(干旱敏感型,在控制和干旱条件下均具有耐旱性)。方法:采用气相色谱-质谱(GC-MS)进行代谢物谱分析,以鉴定不同处理积累差异的化合物。我们应用多元统计方法,如偏最小二乘判别分析(PLS-DA)、层次聚类和途径富集分析,来探索代谢重编程。结果:干旱胁迫诱导了代谢物谱的显著变化,特别是海藻糖、葡萄糖、蔗糖、脯氨酸和缬氨酸等渗透保护剂水平的增加。此外,还观察到有机酸(苹果酸和柠檬酸)和脂肪酸(油酸和亚油酸)的变化。接种PGPR进一步增强了这些代谢反应,增强了渗透调节、活性氧(ROS)解毒和碳氮代谢,RH-725表现出更强的适应性反应。途径富集分析表明,PGPR处理显著影响了淀粉和蔗糖代谢、半乳糖代谢和氨基酸生物合成等代谢途径,这些途径在干旱适应中起着关键作用。结论:这些发现揭示了PGPR如何通过调节关键的生化途径参与芥菜的应激恢复。该研究为已知的PGPR缓解油籽作物干旱胁迫的作用提供了新的分子见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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