Geopropolis from Melipona fasciculata Smith Accelerates Wound Healing in Diabetic Mice.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metabolites Pub Date : 2025-06-19 DOI:10.3390/metabo15060413
Aramys Silva Reis, Gabriel Carvalho de Souza, Guilherme Martins Gomes Fontoura, Luecya Alves de Carvalho Silva, Alberto Jorge Oliveira Lopes, Richard Pereira Dutra, Lucilene Amorim Silva, Rosane Nassar Meireles Guerra, Maria Nilce Sousa Ribeiro, Flávia Raquel Fernandes Nascimento
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引用次数: 0

Abstract

Background: Diabetic foot ulcers present a significant clinical challenge because of their high prevalence and severe complications. The need for innovative and accessible treatment options is critical. Owing to their medicinal properties, natural products, such as geopropolis, hold promise. However, the wound healing potential of the geopropolis of Melipona fasciculata, particularly in accelerating the healing of diabetic ulcers, remains unexplored. In this study, we evaluated the ability of the geopropolis of M. fasciculata to promote wound healing in diabetic mice.

Methods: Geopropolis was collected, prepared as a hydroalcoholic extract, and formulated into a topical cream. Non-obese diabetic (NOD) mice with induced chronic wounds were treated with this cream daily, and wound healing was assessed through macroscopic measurements, histological analysis, cytokine quantification, and in silico molecular docking studies.

Results: The results demonstrated that, compared with the control treatment, the geopropolis cream accelerated wound closure at all the analyzed time points (days 3, 7, and 14), reduced inflammatory infiltrates, and enhanced fibroblast proliferation and collagen deposition. These alterations were particularly pronounced in the final phase of healing, indicating an improvement in wound repair processes. These effects occurred without altering systemic cytokine levels, suggesting a localized treatment action. These results may be partially associated with the theoretical ability of beta-amyrin and cycloartenol to interact with human myeloperoxidase (MPO), as suggested by in silico docking analysis.

Conclusions: Overall, the findings indicate that geopropolis cream could represent a viable alternative for managing diabetic ulcers, providing an effective means to enhance wound healing while remaining accessible to low-income populations.

密缕草蜂胶促进糖尿病小鼠伤口愈合。
背景:糖尿病足溃疡因其高患病率和严重并发症而呈现出显著的临床挑战。提供创新和可获得的治疗方案至关重要。由于其药用特性,天然产品,如土工蜂胶,有希望。然而,其伤口愈合的潜力,特别是在加速愈合糖尿病溃疡,仍未被探索。在这项研究中,我们评估了地蜂胶对糖尿病小鼠伤口愈合的促进作用。方法:收集土工蜂胶,制备成水醇提取物,配制成外用乳膏。非肥胖糖尿病(NOD)小鼠诱导的慢性伤口每天用这种乳膏治疗,通过宏观测量、组织学分析、细胞因子定量和硅分子对接研究来评估伤口愈合情况。结果:结果表明,与对照处理相比,土产蜂胶霜在所有分析时间点(第3、7和14天)加速伤口愈合,减少炎症浸润,增强成纤维细胞增殖和胶原沉积。这些改变在愈合的最后阶段尤其明显,表明伤口修复过程的改善。这些效果发生时没有改变全身细胞因子水平,提示局部治疗作用。这些结果可能部分与β -amyrin和环artenol与人髓过氧化物酶(MPO)相互作用的理论能力有关,正如硅对接分析所表明的那样。结论:总体而言,研究结果表明,geopropolis霜可能是治疗糖尿病溃疡的可行替代方案,提供了有效的手段来促进伤口愈合,同时仍可为低收入人群使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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