Development of a Sustainable Bone Regeneration Material Using Apatite Paste Derived from Eggshell Waste.

IF 5 3区医学 Q1 ENGINEERING, BIOMEDICAL

Journal of Functional Biomaterials Pub Date : 2025-06-01 DOI:10.3390/jfb16060201

Masatsugu Hirota, Chihiro Mochizuki, Toshitsugu Sakurai, Hiroyuki Mishima, Chikahiro Ohkubo, Takatsugu Yamamoto

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引用次数: 0

Abstract

Apatite pastes derived from eggshell waste (BAp) were implanted onto the calvarial bone of rats, and bone formation was evaluated using X-ray μ-computed tomography (CT) and histological evaluation. BAp was mixed with distilled water to prepare a paste. Monoclinic hydroxyapatite of mineral resources (HAp) was used as a control. A 5 mm diameter PTFE (polytetrafluoroethylene) tube was filled with apatite pastes and implanted in the calvarial bone of 9-week-old Sprague Dawley rats for 8 weeks. A larger radiopaque area, similar to that of native bone, was observed in the BAp paste-implanted specimens than that of HAp paste. The bone mineral density (BMD) value of the BAp paste was significantly higher than that of the HAp paste (p < 0.05). In the histological evaluation, new bone formation was noticed from the calvarial side for both apatite specimens, and HAp remained in the PTFE unlike BAp. The bone mass (BM) value of the BAp paste was significantly higher than that of the HAp paste (p < 0.05). SEM and XRD analyses revealed that BAp was microcrystalline and poorly crystalline. The promotion of new bone formation may contribute to the crystallinity and Mg content of BAp. BAp was found to be useful as a bone regeneration material.

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利用蛋壳废料提取的磷灰石膏体开发可持续骨再生材料。

将蛋壳废物磷灰石糊体（BAp）植入大鼠颅骨，采用x射线μ计算机断层扫描（CT）和组织学评价骨形成情况。将BAp与蒸馏水混合制成糊状。以单斜羟基磷灰石（HAp）为对照。采用直径5mm的聚四氟乙烯（PTFE）管填充磷灰石糊剂，植入9周龄Sprague Dawley大鼠颅骨8周。与HAp糊剂相比，BAp糊剂植入标本的不透射线面积更大，与天然骨相似。BAp膏体的骨密度（BMD）值显著高于HAp膏体（p < 0.05）。在组织学评价中，两种磷灰石标本均可见颅骨侧新骨形成，HAp与BAp不同，留在PTFE中。BAp膏体骨质量（BM）值显著高于HAp膏体（p < 0.05）。SEM和XRD分析表明，BAp为微晶，结晶度较差。促进新骨形成可能与BAp结晶度和Mg含量有关。BAp被发现是一种有用的骨再生材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.