Animal model of a bovine pericardial patch for thoracoabdominal aortic aneurysms: step by step.

IF 0.8 Q4 PERIPHERAL VASCULAR DISEASE
Jornal Vascular Brasileiro Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI:10.1590/1677-5449.202401822
Allana Maryel Tobita, Bruno Jeronimo Ponte, Maria Fernanda Cassino Portugal, Anna Paula Weinhardt Baptista, Igor Rafael Sincos, Nelson Wolosker
{"title":"Animal model of a bovine pericardial patch for thoracoabdominal aortic aneurysms: step by step.","authors":"Allana Maryel Tobita, Bruno Jeronimo Ponte, Maria Fernanda Cassino Portugal, Anna Paula Weinhardt Baptista, Igor Rafael Sincos, Nelson Wolosker","doi":"10.1590/1677-5449.202401822","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The treatment of thoracoabdominal aortic aneurysms (TAA) has advanced. Understanding the pathophysiology and surgical approaches to this disease is essential for best therapeutic performance.</p><p><strong>Objectives: </strong>We aimed to improve previously described methods for creating thoracoabdominal aortic aneurysms in a porcine animal model, reducing surgical procedure time and specimen mortality.</p><p><strong>Methods: </strong>A total of 18 swine underwent a surgical procedure to create a TAA. An autologous peritoneal patch was used to create the aneurysm in 2 animals, and a bovine pericardial patch was used in the other 16. The animals were followed up postoperatively, and the aneurysm sac was reexamined after 4 weeks. The animals that did not die in the post-operative period were euthanized according to institutional recommendations.</p><p><strong>Results: </strong>All of the animals underwent laparotomy with retroperitoneal access. Two received an autologous peritoneal patch and 16 received a bovine pericardial patch. Three animals underwent single suprarenal clamping, while 15 underwent sequential clamping. There were no differences in operative time (p<i>=</i>0.207) or total clamping time (p<i>=</i>0.276) between groups. There was a higher mortality rate after 4 weeks in animals that received single clamping (100%) than sequential clamping (26.7%) (p<i>=</i>0.0017).</p><p><strong>Conclusions: </strong>The experimental model of TAA using a bovine pericardial patch and a sequential clamping technique provided a stable and reliable platform that remains stable and patent for up to 4 weeks. This model can be extremely valuable for assessing new endovascular therapy options in living organisms.</p>","PeriodicalId":14814,"journal":{"name":"Jornal Vascular Brasileiro","volume":"24 ","pages":"e20240182"},"PeriodicalIF":0.8000,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186686/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jornal Vascular Brasileiro","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/1677-5449.202401822","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"PERIPHERAL VASCULAR DISEASE","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The treatment of thoracoabdominal aortic aneurysms (TAA) has advanced. Understanding the pathophysiology and surgical approaches to this disease is essential for best therapeutic performance.

Objectives: We aimed to improve previously described methods for creating thoracoabdominal aortic aneurysms in a porcine animal model, reducing surgical procedure time and specimen mortality.

Methods: A total of 18 swine underwent a surgical procedure to create a TAA. An autologous peritoneal patch was used to create the aneurysm in 2 animals, and a bovine pericardial patch was used in the other 16. The animals were followed up postoperatively, and the aneurysm sac was reexamined after 4 weeks. The animals that did not die in the post-operative period were euthanized according to institutional recommendations.

Results: All of the animals underwent laparotomy with retroperitoneal access. Two received an autologous peritoneal patch and 16 received a bovine pericardial patch. Three animals underwent single suprarenal clamping, while 15 underwent sequential clamping. There were no differences in operative time (p=0.207) or total clamping time (p=0.276) between groups. There was a higher mortality rate after 4 weeks in animals that received single clamping (100%) than sequential clamping (26.7%) (p=0.0017).

Conclusions: The experimental model of TAA using a bovine pericardial patch and a sequential clamping technique provided a stable and reliable platform that remains stable and patent for up to 4 weeks. This model can be extremely valuable for assessing new endovascular therapy options in living organisms.

牛胸腹主动脉瘤心包贴片的动物模型:循序渐进。
背景:胸腹主动脉瘤(TAA)的治疗已取得进展。了解这种疾病的病理生理学和手术方法对最佳治疗效果至关重要。目的:我们旨在改进先前描述的在猪动物模型中制造胸腹主动脉瘤的方法,减少手术时间和标本死亡率。方法:共有18头猪接受了外科手术以建立TAA。在2只动物中使用自体腹膜贴片来制造动脉瘤,在另外16只动物中使用牛心包贴片。术后随访,术后4周复查动脉瘤囊。术后未死亡的动物根据机构建议实施安乐死。结果:所有动物均行剖腹手术,经腹膜后通路。2人接受自体腹膜贴片,16人接受牛心包贴片。单次肾上夹持3只,连续夹持15只。两组间手术时间(p=0.207)和总夹紧时间(p=0.276)差异无统计学意义。4周后,单次夹紧组的死亡率(100%)高于连续夹紧组(26.7%)(p=0.0017)。结论:采用牛心包补片和顺序夹持技术的TAA实验模型提供了一个稳定可靠的平台,可保持4周的稳定性和专利性。这个模型对于评估活体血管内治疗的新选择是非常有价值的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Jornal Vascular Brasileiro
Jornal Vascular Brasileiro Medicine-Cardiology and Cardiovascular Medicine
CiteScore
1.20
自引率
0.00%
发文量
57
审稿时长
20 weeks
期刊介绍: The Jornal Vascular Brasileiro is editated and published quaterly to select and disseminate high-quality scientific contents concerning original research, novel surgical and diagnostic techniques, and clinical observations in the field of vascular surgery, angiology, and endovascular surgery. Its abbreviated title is J. Vasc. Bras., which should be used in bibliographies, footnotes and bibliographical references and strips.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信