Transcriptomic Analysis of Venom Secretion in Achelura yunnanensis: Lipid Metabolism, Redox Reactions, and Structural Adaptations.

IF 2.7 2区 农林科学 Q1 ENTOMOLOGY
Insects Pub Date : 2025-06-03 DOI:10.3390/insects16060588
Ping Liu, Hui-Qin Zhu, Si-Ming Wang, Yu-Qian Wang, Zhen-Yuan Ruan, Lu Qiao, Xing-Xing Wu, Qing-Hua Yan, Ya-Ping Lu, Bing Bai, Wei-Feng Ding
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引用次数: 0

Abstract

As a key pest damaging urban greenery in Yunnan, China, Achelura yunnanensis larvae secrete venom for defense, yet the molecular basis of this process remains poorly understood. This study aimed to uncover the molecular mechanisms of venom secretion by comparing the dorsal epidermis tissue (LDET) with the larval proleg tissue (LP). We performed transcriptomic analysis using RNA sequencing to identify differentially expressed genes between LDET and LP (10 biological replicates per tissue type), followed by functional enrichment and gene expression correlation analyses to explore tissue-specific characteristics. LDET exhibited significant upregulation of pathways related to lipid metabolism, redox reactions, and surface protective structure formation, suggesting their roles in venom stabilization, activation, and safe secretion. Conversely, genes linked to non-venom-related functions, such as extracellular matrix organization and epidermal development, were downregulated in LDET, indicating resource reallocation toward venom production. These findings reveal a multi-component mechanism in LDET that supports venom secretion through metabolic and structural adaptations, with lipid metabolism genes constituting 18.3% of total differentially expressed genes, highlighting evolutionary trade-offs in insect defense. This study provides new insights into insect venom secretion and offers potential targets for pest control strategies.

云南梭鲈毒液分泌的转录组学分析:脂质代谢、氧化还原反应和结构适应。
作为破坏云南城市绿化的重要害虫,云南小蠊幼虫分泌毒液进行防御,但其分子机制尚不清楚。本研究旨在通过对背表皮组织(LDET)和幼虫前肢组织(LP)的比较,揭示毒液分泌的分子机制。我们使用RNA测序进行转录组学分析,以鉴定LDET和LP之间的差异表达基因(每种组织类型10个生物重复),然后进行功能富集和基因表达相关性分析,以探索组织特异性特征。LDET显著上调脂质代谢、氧化还原反应和表面保护结构形成相关通路,提示其在毒液稳定、激活和安全分泌中发挥作用。相反,与非毒液相关功能相关的基因,如细胞外基质组织和表皮发育,在LDET中下调,表明资源重新分配给毒液生产。这些发现揭示了LDET的多组分机制,通过代谢和结构适应支持毒液分泌,脂质代谢基因占总差异表达基因的18.3%,突出了昆虫防御的进化权衡。该研究为昆虫毒液分泌提供了新的见解,并为害虫防治策略提供了潜在的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Insects
Insects Agricultural and Biological Sciences-Insect Science
CiteScore
5.10
自引率
10.00%
发文量
1013
审稿时长
21.77 days
期刊介绍: Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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