Functional Study of Opsin Genes in Pardosa astrigera (Araneae: Lycosidae).

IF 2.7 2区 农林科学 Q1 ENTOMOLOGY
Insects Pub Date : 2025-06-05 DOI:10.3390/insects16060595
Shuxin Zhai, Boqi Ren, Xinghua Zhang, Fangyu Shen, Min Ma, Xinmin Li, Rui Li
{"title":"Functional Study of Opsin Genes in <i>Pardosa astrigera</i> (Araneae: Lycosidae).","authors":"Shuxin Zhai, Boqi Ren, Xinghua Zhang, Fangyu Shen, Min Ma, Xinmin Li, Rui Li","doi":"10.3390/insects16060595","DOIUrl":null,"url":null,"abstract":"<p><p>Spiders are important predatory natural enemies in agricultural and forestry ecosystems, yet the role of vision in their predatory behavior remains unclear. In this study, we screened three opsin genes-corresponding to ultraviolet-sensitive and medium-to-long wavelength-sensitive opsins-from the transcriptome sequencing database of <i>Pardosa astrigera</i>. All three genes possess seven transmembrane topological structures and a lysine residue on the second transmembrane domain, which are typical characteristics of opsins. Using quantitative real-time PCR (RT-qPCR), we analyzed the expression patterns of these opsin genes in different tissues, developmental stages, and under the induction of light at three wavelengths. The results showed that all three opsin genes were significantly expressed in the cephalothorax and expressed across developmental stages with no significant differences. Under light induction, their relative expression first increased and then decreased in both male and female adult spiders. Subsequently, RNA interference (RNAi) was used to individually knock down each opsin gene, confirming their involvement in color vision. These results suggest that the three opsin genes are involved in spider vision, laying the foundation for further elucidating the role of vision in spider predation, and offering a new perspective for reducing the unintended killing of natural enemies by insect traps.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193414/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insects","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/insects16060595","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Spiders are important predatory natural enemies in agricultural and forestry ecosystems, yet the role of vision in their predatory behavior remains unclear. In this study, we screened three opsin genes-corresponding to ultraviolet-sensitive and medium-to-long wavelength-sensitive opsins-from the transcriptome sequencing database of Pardosa astrigera. All three genes possess seven transmembrane topological structures and a lysine residue on the second transmembrane domain, which are typical characteristics of opsins. Using quantitative real-time PCR (RT-qPCR), we analyzed the expression patterns of these opsin genes in different tissues, developmental stages, and under the induction of light at three wavelengths. The results showed that all three opsin genes were significantly expressed in the cephalothorax and expressed across developmental stages with no significant differences. Under light induction, their relative expression first increased and then decreased in both male and female adult spiders. Subsequently, RNA interference (RNAi) was used to individually knock down each opsin gene, confirming their involvement in color vision. These results suggest that the three opsin genes are involved in spider vision, laying the foundation for further elucidating the role of vision in spider predation, and offering a new perspective for reducing the unintended killing of natural enemies by insect traps.

黄花伞视蛋白基因的功能研究(蜘蛛目:石蒜科)。
蜘蛛是农林生态系统中重要的天敌,但视觉在其捕食行为中的作用尚不清楚。在本研究中,我们从黄花伞的转录组测序数据库中筛选了三个视蛋白基因,分别对应于紫外线敏感和中长波长敏感的视蛋白。这三个基因都具有7个跨膜拓扑结构,并且在第二跨膜结构域上有赖氨酸残基,这是视蛋白的典型特征。利用实时荧光定量PCR (RT-qPCR)分析了这些视蛋白基因在不同组织、发育阶段以及三种波长光诱导下的表达模式。结果表明,三种视蛋白基因在头胸中均有显著表达,且在不同发育阶段的表达无显著差异。在光照诱导下,其相对表达量先升高后降低。随后,RNA干扰(RNAi)被用于单独敲除每个视蛋白基因,证实它们与色觉有关。这些结果表明,这3种视蛋白基因参与了蜘蛛视觉的调控,为进一步阐明蜘蛛视觉在捕食中的作用奠定了基础,并为减少捕虫器对天敌的意外杀伤提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Insects
Insects Agricultural and Biological Sciences-Insect Science
CiteScore
5.10
自引率
10.00%
发文量
1013
审稿时长
21.77 days
期刊介绍: Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信