A Tyndall Effect-Based Visible Aptasensing Platform Enables Sensitive and Isolation-Free Profiling of Small Extracellular Vesicle Surface Proteins

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-06-24 DOI:10.1021/acs.nanolett.5c01413

Lang Zhang, Yuzhi Xu, Qian Dai, Xinqing Jiang, Maoling Zhu, Bingyang Liu, Xiaoyong Zou, Mo Yang, Xing Han*, Yanfei Zhang*, Wen Yin* and Zong Dai,

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Abstract

Rapid and portable profiling of surface proteins on small extracellular vesicles (sEV) is crucial for noninvasive cancer screening but remains technically challenging. Here, we present a Tyndall effect (TE)-based visible aptasensing platform (TEVAP) for direct, low-cost, and isolation-free detection of sEV surface proteins from complex biological samples. Aptamer-conjugated gold nanoparticles specifically bind to sEV, forming large-scale composites that enhance the TE signal. This enables the identification of five tumor-associated proteins with a detection limit of 6.5 × 10⁶ particles mL^–1 without enzyme catalysis or signal amplification strategies, comparable to other instrument-dependent methods. Applied to clinical samples (e.g., 5 μL of plasma), TEVAP generated distinct signal patterns and effectively distinguished liver and breast cancer patients from healthy controls. With further validation in larger cohorts, this platform holds strong potential for convenient cancer screening and postoperative monitoring.

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基于Tyndall效应的可见适体感测平台可实现细胞外小泡表面蛋白的敏感和无隔离分析。

小细胞外囊泡（sEV）表面蛋白的快速便携分析对于非侵入性癌症筛查至关重要，但在技术上仍然具有挑战性。在这里，我们提出了一个基于Tyndall效应（TE）的可见光适体感应平台（TEVAP），用于直接、低成本、无隔离地检测复杂生物样品中的sEV表面蛋白。适配体共轭金纳米颗粒特异性地与sEV结合，形成大规模复合材料，增强TE信号。这使得鉴定五种肿瘤相关蛋白的检测限为6.5 × 106颗粒mL-1，无需酶催化或信号放大策略，与其他仪器依赖的方法相当。应用于临床样品（如5 μL血浆），TEVAP产生明显的信号模式，可有效区分肝癌和乳腺癌患者与健康对照。在更大的队列中进一步验证，该平台具有方便癌症筛查和术后监测的强大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.