Protective effects of dihydromyricetin against radiation-induced injury to the hematopoietic system

Q1 Health Professions

Radiation Medicine and Protection Pub Date : 2025-06-01 DOI:10.1016/j.radmp.2025.04.004

Xiao Sun , Feifei Xu , Zhiyun Wang , Wenfeng Gou , Yanli Li , Hongying Wu , Yiliang Li , Wenbin Hou

{"title":"Protective effects of dihydromyricetin against radiation-induced injury to the hematopoietic system","authors":"Xiao Sun , Feifei Xu , Zhiyun Wang , Wenfeng Gou , Yanli Li , Hongying Wu , Yiliang Li , Wenbin Hou","doi":"10.1016/j.radmp.2025.04.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the potential protective effects of dihydromyricetin (DHM), a primary bioactive compound derived from <em>Ampelopsis grossedentata</em>, against radiation-induced hematopoietic damage.</div></div><div><h3>Methods</h3><div>The <em>in virto</em> antioxidant capacity of DHM was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assays. Survival rate and hematopoietic damage experiments were conducted on 130 mice. To assess the protective effects of DHM, a lethal dose of 7.5 Gy was delivered to 60 mice, and their 30-d survival rates were assessed and survival time were recorded. The mice were divided into 6 groups in survival analysis: DHM-only (200 mg/kg), IR, IR + low-dose DHM (50 mg/kg), IR + moderate-dose DHM (100 mg/kg), IR + high-dose DHM (200 mg/kg), and IR + amifostine (200 mg/kg). Subsequently, a hematopoietic injury model was established by subjecting 70 mice to whole-body irradiation (WBI) at a dose of 4 Gy. The mice were divided into 7 groups in the hematopoietic damage experiment: control, DHM-only (200 mg/kg), IR, IR + low-dose DHM (50 mg/kg), IR + moderate-dose DHM (100 mg/kg), IR + high-dose DHM (200 mg/kg), and IR + amifostine (200 mg/kg), with 10 mice in each group. The effects of DHM on body weights, blood routine indices, femoral nucleated cell counts, organ indices, and splenic nodules were analyzed. Using hematoxylin and eosin (H&E) staining, the effects of DHM on the spleen and bone marrow were examined. Furthermore, the antioxidant effects of DHM were evaluated by measuring biochemical markers including glutathione (GSH) and superoxide dismutase (SOD).</div></div><div><h3>Results</h3><div>DHM exhibited strong <em>in vitro</em> antioxidant activity (92.17 % in the ABTS assay and 90.75% in the DPPH assay). It significantly improved both the survival time and rates of mice exposed to irradiation at a lethal dose (20% for the IR + low-dose DHM group, 40% for the IR + moderate-dose DHM group, and 50% for the IR + high-dose DHM group; <em>P</em> < 0.05). In the hematopoietic injury experiment, DHM greatly improved blood routine indices, including the white blood cell count and the lymphocyte percentage (<em>P</em> < 0.01). Moreover, DHM considerably increased organ indices, the number of splenic nodules, and the nucleated cell count in the femoral bone marrow. H&E staining revealed that DHM significantly alleviated radiation-induced damage to the spleen and bone marrow. Additionally, DHM treatment greatly enhanced the hepatic GSH and SOD levels of the irradiated mice, reaching 219.01 μmol/g prot and 199.53 U/mg prot, respectively (<em>P</em> < 0.05).</div></div><div><h3>Conclusions</h3><div>Owing to its free radical scavenging potential, DHM can enhance the survival rates of mice exposed to radiation at a lethal dose and mitigate radiation-induced damage to the hematopoietic system. This study serves as a valuable reference for the application of traditional Chinese medicine in radioprotection.</div></div>","PeriodicalId":34051,"journal":{"name":"Radiation Medicine and Protection","volume":"6 3","pages":"Pages 147-155"},"PeriodicalIF":0.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation Medicine and Protection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666555725000450","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

To investigate the potential protective effects of dihydromyricetin (DHM), a primary bioactive compound derived from Ampelopsis grossedentata, against radiation-induced hematopoietic damage.

Methods

The in virto antioxidant capacity of DHM was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assays. Survival rate and hematopoietic damage experiments were conducted on 130 mice. To assess the protective effects of DHM, a lethal dose of 7.5 Gy was delivered to 60 mice, and their 30-d survival rates were assessed and survival time were recorded. The mice were divided into 6 groups in survival analysis: DHM-only (200 mg/kg), IR, IR + low-dose DHM (50 mg/kg), IR + moderate-dose DHM (100 mg/kg), IR + high-dose DHM (200 mg/kg), and IR + amifostine (200 mg/kg). Subsequently, a hematopoietic injury model was established by subjecting 70 mice to whole-body irradiation (WBI) at a dose of 4 Gy. The mice were divided into 7 groups in the hematopoietic damage experiment: control, DHM-only (200 mg/kg), IR, IR + low-dose DHM (50 mg/kg), IR + moderate-dose DHM (100 mg/kg), IR + high-dose DHM (200 mg/kg), and IR + amifostine (200 mg/kg), with 10 mice in each group. The effects of DHM on body weights, blood routine indices, femoral nucleated cell counts, organ indices, and splenic nodules were analyzed. Using hematoxylin and eosin (H&E) staining, the effects of DHM on the spleen and bone marrow were examined. Furthermore, the antioxidant effects of DHM were evaluated by measuring biochemical markers including glutathione (GSH) and superoxide dismutase (SOD).

Results

DHM exhibited strong in vitro antioxidant activity (92.17 % in the ABTS assay and 90.75% in the DPPH assay). It significantly improved both the survival time and rates of mice exposed to irradiation at a lethal dose (20% for the IR + low-dose DHM group, 40% for the IR + moderate-dose DHM group, and 50% for the IR + high-dose DHM group; P < 0.05). In the hematopoietic injury experiment, DHM greatly improved blood routine indices, including the white blood cell count and the lymphocyte percentage (P < 0.01). Moreover, DHM considerably increased organ indices, the number of splenic nodules, and the nucleated cell count in the femoral bone marrow. H&E staining revealed that DHM significantly alleviated radiation-induced damage to the spleen and bone marrow. Additionally, DHM treatment greatly enhanced the hepatic GSH and SOD levels of the irradiated mice, reaching 219.01 μmol/g prot and 199.53 U/mg prot, respectively (P < 0.05).

Conclusions

Owing to its free radical scavenging potential, DHM can enhance the survival rates of mice exposed to radiation at a lethal dose and mitigate radiation-induced damage to the hematopoietic system. This study serves as a valuable reference for the application of traditional Chinese medicine in radioprotection.

查看原文本刊更多论文

二氢杨梅素对辐射致造血系统损伤的保护作用

目的研究从蛇麻中提取的主要生物活性物质二氢杨梅素（DHM）对辐射诱导的造血损伤的保护作用。方法采用1,1-二苯基-2-吡啶肼（DPPH）和2,2 -二氮基-二（3-乙基苯并噻唑-6-磺酸）（ABTS）法测定DHM的体外抗氧化能力。对130只小鼠进行了存活率和造血损伤实验。为评价DHM的保护作用，采用7.5 Gy致死剂量给药60只小鼠，观察其30 d存活率和存活时间。将小鼠分为6组进行生存分析：单剂量DHM （200 mg/kg）、IR、IR +低剂量DHM （50 mg/kg）、IR +中剂量DHM （100 mg/kg）、IR +高剂量DHM （200 mg/kg）、IR +氨磷汀（200 mg/kg）。随后，通过对70只小鼠进行4 Gy剂量的全身照射（WBI），建立了造血损伤模型。造血损伤实验将小鼠分为对照组、单用DHM （200 mg/kg）、IR、IR +低剂量DHM （50 mg/kg）、IR +中剂量DHM （100 mg/kg）、IR +高剂量DHM （200 mg/kg）、IR +氨磷汀（200 mg/kg） 7组，每组10只。分析丹参对大鼠体重、血常规指标、股核细胞计数、脏器指标及脾结节的影响。采用苏木精和伊红（H&；E）染色法观察DHM对脾和骨髓的影响。此外，通过测定谷胱甘肽（GSH）和超氧化物歧化酶（SOD）等生化指标，评价DHM的抗氧化作用。结果dhm具有较强的体外抗氧化活性（ABTS为92.17%，DPPH为90.75%）。它显著提高了致死剂量照射小鼠的存活时间和存活率(IR +低剂量DHM组为20%，IR +中剂量DHM组为40%，IR +高剂量DHM组为50%；P & lt;0.05)。在造血损伤实验中，DHM显著改善了血常规指标，包括白细胞计数和淋巴细胞百分比(P <；0.01)。此外，DHM显著增加器官指数、脾结节数量和股骨髓有核细胞计数。H&；E染色显示DHM明显减轻了辐射引起的脾和骨髓损伤。此外，DHM处理显著提高了辐照小鼠肝脏GSH和SOD水平，分别达到219.01 μmol/g prot和199.53 U/mg prot (P <；0.05)。结论DHM具有清除自由基的能力，可提高致死剂量辐射小鼠的存活率，减轻辐射对造血系统的损伤。本研究为中药在辐射防护中的应用提供了有价值的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊