Combined effects of polystyrene nanoplastics and dinophysistoxin-1 (DTX1) on physiological performance of marine diatom Thalassiosira minima

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-06-24 DOI:10.1016/j.watres.2025.124104

Natnael Sisay Demo , Jiangbing Qiu , Guowang Yan , Ruolin Wu , Mohammad Sadiq Nikzad , Aifeng Li

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Abstract

Currently micro- and nanoplastics (MNPs) and microalgal toxins have become two typical groups of emerging contaminants in various coastal regions worldwide. However, the knowledge about their combined effects on marine organisms is still limited. This study explored the single and combined effects of polystyrene nanoplastics (PSNPs) at 50 mg L^–1 and dinophysistoxin-1 (DTX1) at 1 µmol L^–1 (819 μg l^-1) on the diatom Thalassiosira minima. Results showed that the growth of T. minima was reduced by 22 ± 1.6 %, 53 ± 2.7 %, and 40 ± 2.8 % in DTX1, PSNPs, and their combined treatments, respectively. The maximum adsorption potential of DTX1 on PSNPs reached 78 % after 24 h, which might explain the antagonistic effect of PSNPs and DTX1 on growth and oxidative stress parameters. However, they demonstrated an additive effect on chlorophyll a biosynthesis, nitrogen assimilation, and the silicification of diatoms. The coupling of the silica to nitrogen (Si:N) uptake ratio in diatoms was significantly altered; extracellular dissolved inorganic nitrogen (DIN) in the treatment of PSNPs was reduced by 11.3 ± 4.1 %, while it increased by 47.9 ± 14 % and 60.4 ± 6.9 % in treatments with DTX1 and the combined treatment, respectively. Silicification of T. minima was reduced by 50 ± 5.9 % and 49 ± 1.8 % in treatments with DTX1 alone and the combined treatment, respectively. These findings suggest that DTX1 exposure results in an increase in N uptake and a decrease in Si uptake, demonstrating an inverse effect on the uptake of nutrients. Transcriptome analysis revealed downregulation of some amino acid genes possibly accounts for this change. This study is significant for understanding the combined effects of microplastics and phycotoxins on diatoms.

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聚苯乙烯纳米塑料和藻毒素-1 （DTX1）对海洋硅藻最小海藻生理性能的联合影响

目前，微纳塑料和微藻毒素已成为世界各地沿海地区两类典型的新兴污染物。然而，关于它们对海洋生物的综合影响的知识仍然有限。研究了50 mg L-1聚苯乙烯纳米塑料（PSNPs）和1 μ mol L-1 dinophysitoxin -1 (DTX1) （819 μg L-1）对最小硅藻（thalassisira minima）的单独和联合作用。结果表明，DTX1、PSNPs及其联合处理分别使小褐飞虱的生长降低22±1.6%、53±2.7%和40±2.8%。24 h后DTX1对PSNPs的最大吸附势达到78%，这可能解释了PSNPs和DTX1对生长和氧化应激参数的拮抗作用。然而，它们对叶绿素a的生物合成、氮同化和硅藻的硅化具有加性作用。硅藻对硅氮（Si:N）吸收比的耦合作用显著改变；细胞外溶解无机氮（DIN）在psnp处理下降低了11.3±4.1%，而DTX1和联合处理分别增加了47.9±14%和60.4±6.9%。单用DTX1和联合使用DTX1分别可降低T. minima硅化率50±5.9%和49±1.8%。这些发现表明，DTX1暴露导致氮吸收增加和硅吸收减少，表明对营养物质吸收的反作用。转录组分析显示，一些氨基酸基因的下调可能解释了这种变化。本研究对了解微塑料和藻毒素对硅藻的综合影响具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.