[Protective effects of 5-n-alkylresorcinols on H_2O_2-induced oxidative damage in Caco-2 cells].

Abstract

Objective: To investigate the protective effects of five alkylresorcinol homologs on hydrogen peroxide(H_2O_2)-induced oxidative damage in human colorectal adenocarcinoma cells(Caco-2).

Methods: The H_2O_2-induced oxidative damage of Caco-2 cells was used as a model, and the survival rate of Caco-2 cells pretreated with different concentrations(0-80 μmol/L)of 5-n-alkylresorcinols was determined to determine the optimal concentration range. The protective effects of 5-n-alkylresorcinols on oxidative-damaged cells was evaluated by measuring the levels of reactive oxygen species(ROS), malondialdehyde(MDA), glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and catalase(CAT) activities.

Results: 5-n-alkylresorcinols had no obvious toxic effect on Caco-2 cells in the concentration range of 5-80 μmol/L. The activities of GSH-Px, SOD and CAT oxidase in cell culture supernatant and cells were significantly lower than those in the control group after 4 hours of 600 μmol/L H_2O_(2 )treatment(P<0.05). The addition of 5 alkylresorcinol homologues at different concentrations alleviated the damage of H_2O_2 to different degrees, and the ROS level and MDA content decreased compared with the modularity, and the activity of GSH-Px, SOD and CAT was restored to a certain extent.

Conclusion: 5-n-alkylresorcinols can protect Caco-2 cells from oxidative damage to H_2O_2 by activating intracellular antioxidant enzyme activity and inhibiting lipid peroxidation levels.