[Expression of LINC 00478 in oral squamous cell carcinoma and its effect on proliferation, migration and invasion].

Q4 Medicine

上海口腔医学 Pub Date : 2025-04-01

Qian Zhao, Linzi Han, Lu Zhao, Lili Wang, Likun Zhuang, Rongtao Yuan

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引用次数: 0

Abstract

Puepose: To examine the expression difference of LINC 00478 in oral squamous cell carcinoma (OSCC) and analyze its relationship with the prognosis of OSCC patients. To investigate the effects of LINC 00478 on proliferation, migration, and invasion of OSCC cells, with the goal of offering a new potential approach for molecular detection and clinical diagnosis of OSCC.

Methods: Transcriptome data samples from OSCC were obtained from TCGA, and R software was utilized to assess the expression variance of LINC 00478 in OSCC tumor and normal tissues. Tissue samples from 26 OSCC patients, including cancer and adjacent tissues, were collected for RT-PCR analysis to determine the differential expression of LINC 00478 in OSCC tissues. In vitro experiments were conducted to explore the impact of LINC 00478 on proliferation, invasion, and migration of CAL27 cells. CCK-8 assay was used to observe the proliferation capability of LINC 00478 in CAL27 cells, while Transwell experiment was employed to assess its effects on cell migration and invasion. GraphPad Prism 8.0.2 software was used for statistical analysis of the data.

Results: Analysis of the TCGA database revealed that LINC 00478 was weakly expressed in OSCC tissues compared to adjacent normal tissues (P＜0.001), with high-expression LINC 00478 correlating with improved patient prognosis(P＜0.05). Furthermore, LINC 00478 was significantly under-expressed in 26 OSCC tissues (P＜0.001). Experimental data from CCK-8 assays indicated that upregulation of LINC 00478 suppressed CAL27 proliferation (P＜0.05). Additionally, Transwell experiments demonstrated that high-expression LINC 00478 significantly inhibited CAL27 migration and invasion (P＜0.05).

Conclusions: LINC 00478 is significantly under-expressed in OSCC, and its high expression is positively correlated with a better prognosis. Over-expression of the LINC 00478 gene has been shown to inhibit the proliferation, migration, and invasion of CAL27 cells, suggesting a potential role in inhibiting cancer progression in OSCC.

本刊更多论文

LINC 00478在口腔鳞状细胞癌中的表达及其对增殖、迁移和侵袭的影响

目的：探讨LINC 00478在口腔鳞状细胞癌（OSCC）中的表达差异，并分析其与预后的关系。探讨LINC 00478对OSCC细胞增殖、迁移和侵袭的影响，为OSCC的分子检测和临床诊断提供一种新的潜在方法。方法：从TCGA获取OSCC的转录组数据样本，利用R软件评估LINC 00478在OSCC肿瘤和正常组织中的表达差异。收集26例OSCC患者的组织样本，包括癌及癌旁组织，进行RT-PCR分析，确定LINC 00478在OSCC组织中的差异表达。通过体外实验探讨LINC 00478对CAL27细胞增殖、侵袭和迁移的影响。采用CCK-8法观察LINC 00478在CAL27细胞中的增殖能力，采用Transwell实验评估其对细胞迁移和侵袭的影响。采用GraphPad Prism 8.0.2软件对数据进行统计分析。结果：TCGA数据库分析显示，与癌旁正常组织相比，LINC 00478在癌旁组织中表达较弱（P<0.001），高表达的LINC 00478与患者预后改善相关（P<0.05）。此外，LINC 00478在26个OSCC组织中显著低表达（P<0.001）。CCK-8实验数据显示，上调LINC 00478可抑制CAL27的增殖（P<0.05）。Transwell实验表明，高表达的LINC 00478显著抑制CAL27的迁移和侵袭（P<0.05）。结论：LINC 00478在OSCC中显著低表达，其高表达与预后良好呈正相关。研究显示，LINC 00478基因的过表达可抑制CAL27细胞的增殖、迁移和侵袭，提示其在OSCC中具有抑制肿瘤进展的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

上海口腔医学 Medicine-Medicine (all)

CiteScore

0.30

自引率

0.00%

发文量

5299

期刊介绍： "Shanghai Journal of Stomatology (SJS)" is a comprehensive academic journal of stomatology directed by Shanghai Jiao Tong University and sponsored by the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. The main columns include basic research, clinical research, column articles, clinical summaries, reviews, academic lectures, etc., which are suitable for reference by clinicians, scientific researchers and teaching personnel at all levels engaged in oral medicine.