Long Non-Coding RNA n336928 Facilitates the Progression of Pancreatic Neuroendocrine Neoplasms via NF-κB Pathway Mediated by EZH2.

Abstract

Pancreatic neuroendocrine neoplasms (pNENs) are among the most frequently occurring neuroendocrine neoplasms (NENs). In this study, we aimed to investigate the role and impact of LncRNA-n336928 (n336928) in pNENs. We evaluated changes in cell proliferation using CCK-8, colony formation, and EDU assays, and assessed the effects on cell migration and invasion through transwell assays. RNA-seq analysis was performed to explore the potential mechanisms underlying n336928-induced pNEN progression. Subcutaneous tumorigenesis models were constructed to verify the function of n336928 in vivo. Our findings indicate that overexpression of n336928 significantly accelerated the proliferation, migration, and invasion of pNEN cell lines, while concurrently suppressing apoptosis. Furthermore, transcriptome sequencing revealed that the NF-κB pathway was activated in pNEN cells following n336928 overexpression. We have established that the upregulation of n336928 promotes the progression of pNENs via the NF-κB signaling pathway, thereby implicating it in the occurrence and development of pNENs.