Antimicrobial, Time-Kill Kinetics, and Biofilm Inhibition Properties of Diospyros lycioides Chewing Stick Used in Namibia Against Enterococcus faecalis.

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Journal of Tropical Medicine Pub Date : 2025-06-16 eCollection Date: 2025-01-01 DOI:10.1155/jotm/7544856
Albertina Mariina Ndinelao Shatri, Silas Kudakwashe Bere, Denise Bouman, Davis Ropafadzo Mumbengegwi
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引用次数: 0

Abstract

Background: Medicinal plants are used in Namibia for oral hygiene and to treat oral diseases. Validating the content and efficacy of medicinal chewsticks used in communities helps to provide proof of concept of medicinal plants used as a complementary/alternative medicine for oral diseases. Aim: This study presents the first report on quantified phytoconstituents, antimicrobial, time-kill kinetics, and biofilm inhibition properties of Diospyros lycioides organic and aqueous extracts against Enterococcus faecalis. Methodology: Dry plant materials were ground into powder and macerated in methanol and distilled water. Different phytoconstituents were quantified by Folin-Ciocalteu colorimetric method, ferric reducing antioxidant power assay, and DPPH free radical scavenging. An antibacterial assay was performed using the agar well diffusion method and a resazurin 96-well-based assay. Kill-time assay was done at various concentrations over 4 h. Biofilm inhibition was done using the crystal violet method. Results: Higher total flavonoid, total phenol contents, and free radical scavenging abilities were reported in methanol twig extracts. Inhibition zones of 28 ± 0.82 mm, with MICs of 15.6 ± 0.00 μg/mL, are reported against E. faecalis. The bactericidal endpoint of D. lycioides organic extracts for E. faecalis was reached after 4 h of incubation at 8 × MIC (124.8 μg/mL). These were comparable to the positive control, gentamicin. The organic extracts showed ≥ 50% biofilm inhibition against root canal-infecting E. faecalis at concentrations between 7.8 and 500 μg/mL, indicating strong biofilm inhibition. Conclusion: The study demonstrated that D. lycioides crude extracts have promising antibacterial properties and can eradicate E. faecalis biofilms in root canal treatments.

在纳米比亚使用的拟石弧菌咀嚼棒对粪肠球菌的抗菌、时效动力学和生物膜抑制性能。
背景:药用植物在纳米比亚用于口腔卫生和治疗口腔疾病。验证社区中使用的药用咀嚼棒的成分和功效有助于证明药用植物作为口腔疾病的补充/替代药物的概念。目的:本文首次报道了水提物和水提物对粪肠球菌的植物成分、抗菌性能、时间动力学和生物膜抑制性能的定量研究。方法:将干燥的植物材料磨成粉末,在甲醇和蒸馏水中浸泡。采用Folin-Ciocalteu比色法、铁还原抗氧化能力测定和DPPH自由基清除能力测定不同植物成分。采用琼脂孔扩散法和reazurin 96孔法进行抑菌试验。杀伤时间测定在不同浓度下超过4小时。采用结晶紫法进行生物膜抑制。结果:甲醇嫩枝提取物具有较高的总黄酮、总酚含量和自由基清除能力。对粪肠杆菌的抑制范围为28±0.82 mm, mic为15.6±0.00 μg/mL。8 × MIC (124.8 μg/mL)作用4 h后,枸杞有机提取物对粪肠杆菌的杀菌作用达到终点。这些与阳性对照庆大霉素相当。在浓度为7.8 ~ 500 μg/mL范围内,有机提取物对根管感染的粪肠杆菌的生物膜抑制作用≥50%,显示出较强的生物膜抑制作用。结论:本研究表明,lyciide粗提物具有良好的抗菌性能,可根治根管治疗中粪肠球菌生物膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Tropical Medicine
Journal of Tropical Medicine Immunology and Microbiology-Parasitology
CiteScore
3.90
自引率
4.50%
发文量
0
审稿时长
14 weeks
期刊介绍: Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
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