Kidney damage following a 90-day subchronic inhalation exposure to HTP aerosol in SD rats.

IF 2.1 4区医学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Drug and Chemical Toxicology Pub Date : 2025-06-24 DOI:10.1080/01480545.2025.2513693

Yushan Tian, Hongjuan Wang, Yaning Fu, Wenming Wang, Shuhao Ma, Xiaoxiao Xu, Xianmei Li, Fengjun Lu, Pengxia Feng, Shulei Han, Huan Chen, Hongwei Hou, Qingyuan Hu

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引用次数: 0

Abstract

Cigarette smoking (CS) was reported to induce the risk of renal diseases. However, heated tobacco product (HTP), a modified risk tobacco product, was claimed to reduce exposure risk, its health risk of kidney was still unclear. In this study, subchronic inhalation toxicity of HTP aerosols for 90 days was performed to assess its health risk of kidney. The nose-only exposure experiments were performed with SD rats. All the rats were randomly divided into sham, HTP (HTP_10, HTP_23, and HTP_50), and CS (Cig_23) groups. After exposure, the blood and kidney were prepared to detect its redox state, biomarkers in the early injury, apoptosis, and histopathology. The results showed that HTP and cigarette smoke both induced the expression of biomarkers including kidney injury molecule-1, neutrophil gelatinase-associated lipocalin, interleukin 18, and oxidative stress. For biochemical markers associated with kidney function, CS induced increased creatinine and urea for female rats and uric acid decreased, while HTP exposure only induced the upregulation of creatinine. Moreover, no obvious apoptosis and pathology of kidney were observed after HTP exposure, which indicated that HTP exposure may induce some biomarkers in the early stage of kidney injury without more serious changes. Overall, these results suggested that HTP with high concentration exposure showed potential slight health risks of kidney.

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吸烟（CS）可引起肾脏疾病的危险。然而，加热烟草制品（HTP）作为一种改良的风险烟草制品，虽然声称可以降低暴露风险，但其对肾脏的健康风险尚不清楚。本研究对HTP气溶胶进行了90天的亚慢性吸入毒性试验，以评估其肾脏健康风险。用SD大鼠进行单鼻暴露实验。将大鼠随机分为假手术组、HTP组（HTP_10、HTP_23、HTP_50）和CS组（Cig_23）。暴露后，制备血液和肾脏，检测其氧化还原状态、早期损伤、细胞凋亡和组织病理学中的生物标志物。结果表明，HTP和香烟烟雾均诱导了肾损伤分子-1、中性粒细胞明胶酶相关脂钙素、白细胞介素18和氧化应激等生物标志物的表达。对于与肾功能相关的生化指标，CS诱导雌性大鼠肌酐和尿素升高，尿酸降低，而HTP暴露仅诱导肌酐升高。此外，HTP暴露后未观察到明显的肾脏凋亡和病理变化，这表明HTP暴露可能在肾脏损伤早期诱导了一些生物标志物，但没有更严重的变化。总的来说，这些结果表明高浓度暴露的HTP对肾脏有潜在的轻微健康风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug and Chemical Toxicology 医学-毒理学

CiteScore

6.00

自引率

3.80%

发文量

审稿时长

3 months

期刊介绍： Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.