Chemo-Enzymatic Synthesis of Viscoelastic Pseudopeptidoglycan Networks.

IF 4 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Bioconjugate Chemistry Pub Date : 2025-06-24 DOI:10.1021/acs.bioconjchem.5c00014

Philipp Loibner, David Bučak-Gasser, Katharina Schober, Tobias Steindorfer, Monika Brandtner, Tobias Dorn, Tanja Wrodnigg, Dmytro Neshchadin, Georg Gescheidt-Demner, Matej Bračič, Florian Lackner, Tamilselvan Mohan, Karin Stana Kleinschek, Rupert Kargl

引用次数: 0

Abstract

Bacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and ¹H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response.

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粘弹性假多肽聚糖网络的化学酶合成。

细菌肽聚糖（PGs）是抗生素和免疫细胞的重要靶点。半合成合成pg的化学方法既乏味又浪费。在这项工作中，我们描述了一种利用蛋白酶木瓜蛋白酶和与聚糖结合的定制肽形成伪pg （PPGs）的新方法。形成动力学由流变学和1H NMR监测。根据温度和聚糖链之间肽桥的数量，形成了强度可控的粘弹性凝胶。我们认为这种新方法对生物材料研究有很大的影响，因为它可以用于传递肽，测试抗生素功效，或研究人体免疫细胞反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioconjugate Chemistry 生物-化学综合

CiteScore

9.00

自引率

2.10%

发文量

236

审稿时长

1.4 months

期刊介绍： Bioconjugate Chemistry invites original contributions on all research at the interface between man-made and biological materials. The mission of the journal is to communicate to advances in fields including therapeutic delivery, imaging, bionanotechnology, and synthetic biology. Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of bioconjugates, including the preparation, properties and applications of biomolecular conjugates.