NUTF2 Promotes Proliferation of Oral Squamous Cell Carcinoma Through PI3K/AKT Signaling Pathway.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-08-01 Epub Date: 2025-06-22 DOI:10.1111/odi.15326

Zhihong Chen, Zhenyuan Zhao, Teng Xu, Feng Zou, Yuemei Yang, Peng Lu, Yiren Chen, Zaiou Zhu, Yao He, Yunong Wu, Xu Ding, Xiaomeng Song

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引用次数: 0

Abstract

Background: Oral squamous cell carcinoma (OSCC) is associated with a poor prognosis. Nuclear transport factor 2 (NUTF2) participates in nucleoprotein transport. However, the mechanism of NUTF2 in OSCC remains unclear.

Methods: We conducted a retrospective study in which patients had not received preoperative chemoradiotherapy and all oral lesions were primary. We assessed the NUTF2 expression in OSCC patients' tissues by polymerase chain reaction and western blot. Immunohistochemical staining was performed based on 203 OSCC specimens to analyze the characteristics of NUTF2 expression. Furthermore, we studied the impact of NUTF2 on OSCC cells through a CCK-8, wound healing, transwell, and flow cytometry assays. The mechanism of NUTF2 was further investigated through western blot. The effect of NUTF2 on tumor growth was confirmed in vivo.

Results: NUTF2 was upregulated in OSCC tissues and related to the poor prognosis. The overexpression of NUTF2 promoted OSCC cell growth in CCK-8 and wound healing. Moreover, our results indicated that the AKT signaling pathway mediated the promoting role of NUTF2 in OSCC. These results were verified in subcutaneous neoplasia in vivo.

Conclusion: Our study highlights the promoting effect of NUTF2 on OSCC proliferation and suggests its potential as a therapeutic target for molecular therapy of OSCC.

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NUTF2通过PI3K/AKT信号通路促进口腔鳞状细胞癌增殖

背景：口腔鳞状细胞癌（OSCC）预后不良。核转运因子2 （NUTF2）参与核蛋白的转运。然而，NUTF2在OSCC中的作用机制尚不清楚。方法：我们进行了一项回顾性研究，所有患者术前未接受放化疗，所有口腔病变都是原发的。采用聚合酶链反应和western blot方法检测NUTF2在OSCC患者组织中的表达。对203例OSCC标本进行免疫组化染色，分析NUTF2的表达特征。此外，我们通过CCK-8、伤口愈合、transwell和流式细胞术检测研究了NUTF2对OSCC细胞的影响。western blot进一步研究NUTF2的作用机制。体内实验证实了NUTF2对肿瘤生长的影响。结果：NUTF2在OSCC组织中表达上调，与预后不良有关。NUTF2的过表达促进了CCK-8中OSCC细胞的生长和伤口愈合。此外，我们的研究结果表明AKT信号通路介导了NUTF2在OSCC中的促进作用。这些结果在体内皮下肿瘤中得到证实。结论：我们的研究突出了NUTF2对OSCC增殖的促进作用，提示其可能作为OSCC分子治疗的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.