Effectiveness of steam cleaning technology on reducing the occurrence of ESKAPE organisms and Escherichia coli in the stables of an equine referral hospital

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-06-21 DOI:10.1016/j.jevs.2025.105636

S. Sosa-Portugal , L. Dale , J. Devaney , A. Sharp , F. Malalana , D. Timofte

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引用次数: 0

Abstract

Background

The “ESKAPE” bacteria include a group of organisms known for their multidrug resistance and potential association with nosocomial infections in human and veterinary hospitals.

Aims/objectives

This study aimed to evaluate the effectiveness of steam cleaning in reducing the number of ESKAPE organisms on environmental surfaces from the stables of an equine veterinary referral hospital.

Methods

Environmental sampling was performed at two timepoints (pre- and post-steam cleaning) on smooth metallic doors and rough rubber stable walls. Microbiological culture targeted ESKAPE organisms and Escherichia coli. Adenosine triphosphate (ATP) bioluminescence swabs were taken concurrently to compare their readings in relation to ESKAPE presence, and settle plates were used to assess aerosolised contamination. A selection of isolates obtained post-steam cleaning underwent whole genome sequencing (WGS).

Results

Steam cleaning led to a significant reduction in ESKAPE organisms on smooth metal doors compared to rough rubber walls (52 % vs. 10 %, p- 0.004, 95 % CI). ATP swabs provided a quick indicator for cleanliness, however Spearman’s correlation coefficient (ρ) failed to find a significant correlation with microbiological culture results (ρ = 0.082, p-0.467). No ESKAPE organisms were isolated from settle plates at any timepoint. WGS of ESKAPE isolates obtained post-steam cleaning (n = 17) identified resistance genes to at least four classes of antimicrobials in all isolates, while some isolates also carried “qacE” resistance genes to quaternary ammonium compounds (n = 6).

Conclusion

This study provides novel data suggesting that steam cleaning was effective at reducing ESKAPE organisms on smooth surfaces and may offer an environmentally friendly alternative to chemical disinfectants.

查看原文本刊更多论文

蒸汽清洗技术对减少某马转诊医院马厩内ESKAPE微生物和大肠杆菌发生的效果

背景：“ESKAPE”细菌包括一组以其多药耐药性和与人类和兽医医院院内感染的潜在关联而闻名的生物体。目的/目的：本研究旨在评估蒸汽清洗在减少马兽医转诊医院马厩环境表面上ESKAPE生物数量的有效性。方法：在两个时间点（蒸汽清洗前和蒸汽清洗后）对光滑的金属门和粗糙的橡胶稳定壁进行环境采样。微生物培养针对ESKAPE生物和大肠杆菌。同时采用三磷酸腺苷（ATP）生物发光拭子比较其与ESKAPE存在的读数，并使用沉淀板评估雾化污染。选择蒸汽清洗后获得的分离株进行全基因组测序（WGS）。结果：与粗糙的橡胶墙相比，蒸汽清洗导致光滑金属门上ESKAPE生物的显著减少（52% vs. 10%, p- 0.004, 95% CI）。ATP拭子提供了清洁度的快速指标，但Spearman相关系数（ρ）未能发现与微生物培养结果的显著相关性（ρ=0.082, p-0.467）。在任何时间点均未从沉降板中分离出ESKAPE生物。蒸汽清洗后获得的ESKAPE分离株（n=17）的WGS鉴定出所有分离株对至少4类抗菌素的耐药基因，而部分分离株还携带对季铵化合物的“qacE”耐药基因（n=6）。结论：该研究提供了新的数据，表明蒸汽清洗可有效减少光滑表面上的ESKAPE生物，可能是化学消毒剂的环保替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.