Dental pulp enhances dentin bonding durability: Evidence from a rat model

IF 4.8 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-06-20 DOI:10.1016/j.jdent.2025.105925

Bing-Jie Zhong , Jing-Hui Lin , Zheng-Xing Lin , Zhi-Cen Lu , Deng-Wei Hong , Hao Yu

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引用次数: 0

Abstract

Objectives

To develop a novel animal model for investigating dentin bonding and to examine how dental pulp vitality affects the long-term stability of dentin-resin bonds.

Methods

1) A split-mouth design was employed in Sprague-Dawley rats. Mandibular first molars were assigned to the vital or nonvital group (n = 6). In vital teeth, 0.3 mm of the mesial surface was removed to expose the dentin, followed by the application of a self-etch adhesive and light-cured resin composite. For nonvital teeth, root canal treatment was performed before the same bonding procedure. Micro-CT analysis and hematoxylin-eosin staining were conducted for model validation. 2) A total of 116 rats were used for dentin bonding evaluation. The composite survival rates, microshear bond strength (μSBS), and interfacial structure were characterized at 0, 2, 4, and 6 weeks (with 29 rats sacrificed at each interval) via field emission scanning electron microscopy, atomic force microscopy, and confocal laser scanning microscopy. Additional biochemical analysis of bonded dentin (n = 3) was performed via data-independent acquisition mass spectrometry.

Results

1) The animal model was validated successfully, with micro-CT and histology confirming that there were no pathological alterations in pulp or periapical tissues. 2) Vital teeth exhibited superior bonding durability, with significantly higher survival rates, stable μSBS values, and excellently characterized interface. Nonvital teeth exhibited decreased bond strength, microcracks, poor sealing, reduced mechanical properties, and increased matrix metalloproteinase (MMP) activity. Proteomic analysis suggested that pulp vitality regulates MMP expression, preserving interfacial stability.

Conclusions

Dental pulp vitality enhances bonding durability by maintaining interface integrity and modulating endogenous enzymes, particularly MMPs.

Clinical relevance

The protective role of dental pulp vitality in stabilizing the dentin-resin interface and suppressing MMP activity may lead to the development of novel dentin bonding strategies.

Abstract Image

查看原文本刊更多论文

牙髓增强牙本质粘合耐久性：来自大鼠模型的证据。

目的：建立一种新的动物模型来研究牙本质结合，并研究牙髓活力如何影响牙本质-树脂结合的长期稳定性。方法：1)Sprague-Dawley大鼠采用开口设计。下颌第一磨牙分为生命组和非生命组（n=6）。在生牙中，去除0.3 mm的中表面以暴露牙本质，然后应用自蚀刻粘合剂和光固化树脂复合材料。对于非重要牙齿，在相同的粘接程序之前进行根管治疗。显微ct分析和苏木精-伊红染色进行模型验证。2)采用116只大鼠进行牙本质结合评价。采用场发射扫描电镜、原子力显微镜和激光共聚焦扫描显微镜，分别在0、2、4和6周（每个间隔29只大鼠）观察复合存活率、微剪切结合强度（μSBS）和界面结构。结合牙本质（n=3）的生化分析通过数据独立采集质谱进行。结果：1)动物模型验证成功，显微ct及组织学检查证实牙髓及根尖周组织未见病理改变。2)活牙具有良好的粘接耐久性，存活率显著提高，μSBS值稳定，界面表征良好。非生命牙齿表现出粘结强度下降、微裂纹、密封性差、机械性能降低和基质金属蛋白酶（MMP）活性增加。蛋白质组学分析表明，牙髓活力调节MMP的表达，保持界面稳定性。结论：牙髓活力通过维持界面完整性和调节内源性酶（尤其是MMPs）来增强牙髓结合的耐久性。临床意义：牙髓活力在稳定牙本质-树脂界面和抑制MMP活性方面的保护作用可能导致新的牙本质结合策略的发展。

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.