Pathogenicity of Arcobacter cryaerophilus in two human intestinal cell lines.

IF 4 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2025-06-22 DOI:10.1186/s13099-025-00721-4

Antonia Bachus, Sarah Beyer, Roland Bücker, Soroush Sharbati, Thomas Alter, Greta Gölz

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Abstract

Background: Arcobacter cryaerophilus is considered an emerging foodborne pathogen and is associated primarily with infectious gastrointestinal disease in humans. However, the underlying pathogenic mechanisms remain poorly understood. Therefore, the aim of the present study was to investigate the pathogenic potential of twelve A. cryaerophilus strains using various in vitro assays in two human colonic cell lines, HT-29/B6 and T84.

Results: All strains tested were able to adhere to and invade into both cell lines, with strain-dependent differences in their adhesion and invasion rates. In addition, two strains showed cytotoxic effects on both cell lines. The ability to disrupt the epithelial barrier function of T84 cell monolayers was shown for two strains by measurement of transepithelial electrical resistance. As structural factors correlate with the barrier dysfunction, immunofluorescence staining of the tight junction domain was performed, and revealed an altered distribution of claudin-5 in infected cells.

Conclusions: The results highlight the strain-dependent pathogenic mechanisms of A. cryaerophilus that may contribute to key symptoms such as diarrhoea. These findings also highlight the importance of further research into the pathogen A. cryaerophilus.

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嗜冷杆菌在两种人肠细胞系中的致病性。

背景：嗜冷杆菌被认为是一种新兴的食源性病原体，主要与人类感染性胃肠道疾病有关。然而，潜在的致病机制仍然知之甚少。因此，本研究的目的是通过不同的体外实验，在HT-29/B6和T84两种人结肠细胞系中研究12株嗜冷芽孢杆菌的致病性。结果：所有菌株均能粘附并侵入两种细胞系，其粘附率和侵入率存在菌株依赖性差异。此外，两株菌株对两种细胞系均有细胞毒作用。通过测量两株T84细胞的上皮电阻，显示了破坏T84细胞单层上皮屏障功能的能力。由于结构因素与屏障功能障碍相关，我们对紧密连接结构域进行了免疫荧光染色，结果显示claudin-5在感染细胞中的分布发生了改变。结论：结果强调了嗜冷芽孢杆菌菌株依赖的致病机制，可能导致腹泻等关键症状。这些发现也强调了进一步研究嗜冷芽孢杆菌的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).