Effect of different feeding times on the structure of the intestinal flora of Rana dybowskii tadpole.

IF 1.7 4区医学 Q2 BIOLOGY

Chronobiology International Pub Date : 2025-08-01 Epub Date: 2025-06-23 DOI:10.1080/07420528.2025.2519308

Wandi Xu, Hongyue Shi, Yingdong Li

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Abstract

Feeding time is a critical factor influencing the growth, feeding efficiency, and health of animals. This study aimed to investigate the impact of different feeding times on the gut microbiome composition in Rana dybowskii tadpoles over a 50-d period. Using 16S rRNA sequencing, we analyzed the gut microbiome at four feeding times: 6:00, 12:00, 18:00, and 24:00. Our results revealed that tadpoles fed at 12:00 and 18:00 grew significantly faster than those fed at 6:00 and 24:00. The highest microbial abundance and diversity were observed in the 12:00 feeding group. Additionally, three probiotic bacteria-Akkermansia muciniphila, Bacteroides ovatus, and Butyricicoccus pullicaecorum-were identified as dominant species in this group. These findings suggest that feeding time not only influences the composition and structure of the intestinal microbiota in R. dybowskii tadpoles but also affects their growth performance. These results highlight the importance of feeding time in improving tadpole health and farming efficiency.

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不同摄食时间对小蝌蚪肠道菌群结构的影响。

饲养时间是影响动物生长、饲养效率和健康的重要因素。本研究旨在研究不同摄食时间对戴氏蛙蝌蚪肠道微生物组成的影响，为期50 d。通过16S rRNA测序，我们分析了4个饲喂时间（6:00、12:00、18:00和24:00）的肠道微生物组。结果表明，12:00和18:00采食的蝌蚪生长速度明显快于6:00和24:00采食的蝌蚪。12:00饲喂组的微生物丰度和多样性最高。此外，3种益生菌——嗜粘杆菌、卵形拟杆菌和白斑丁酸梭菌被鉴定为优势种。由此可见，摄食时间不仅会影响小蝌蚪肠道菌群的组成和结构，还会影响小蝌蚪的生长性能。这些结果强调了饲养时间对改善蝌蚪健康和养殖效率的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chronobiology International 生物-生理学

CiteScore

5.60

自引率

7.10%

发文量

110

审稿时长

1 months

期刊介绍： Chronobiology International is the journal of biological and medical rhythm research. It is a transdisciplinary journal focusing on biological rhythm phenomena of all life forms. The journal publishes groundbreaking articles plus authoritative review papers, short communications of work in progress, case studies, and letters to the editor, for example, on genetic and molecular mechanisms of insect, animal and human biological timekeeping, including melatonin and pineal gland rhythms. It also publishes applied topics, for example, shiftwork, chronotypes, and associated personality traits; chronobiology and chronotherapy of sleep, cardiovascular, pulmonary, psychiatric, and other medical conditions. Articles in the journal pertain to basic and applied chronobiology, and to methods, statistics, and instrumentation for biological rhythm study. Read More: http://informahealthcare.com/page/cbi/Description