Development of Efficiently Quantitative Analysis Targeting α-Galactosylceramide in FreeStyle 293-F Cells.

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Chemical & pharmaceutical bulletin Pub Date : 2025-01-01 DOI:10.1248/cpb.c25-00161

Kentaro Iwata, Koji Nishimura, Kanae Otsutomo, Yasunori Oba, Kei Motonaga

引用次数: 0

Abstract

α-Galactosylceramide (α-GalCer), a synthetic lipid that activates natural killer T cells, has been studied for administration as an active component of artificial adjuvant vector cells (aAVCs) for cancer therapy. A quantification method for α-GalCer content in the cells is essential to ensure the antitumor effect of aAVCs. In this study, a new analytical procedure was established using LC and tandem MS, with a lipid extraction method and internal standard method, and its analytical validation was performed. Furthermore, the procedure was applied to determine α-GalCer content in α-GalCer-loaded model cells, which are the original vector cells in aAVCs.

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自由式293-F细胞α-半乳糖神经酰胺高效定量分析的进展

α-半乳糖神经酰胺（α-GalCer）是一种激活自然杀伤T细胞的合成脂质，已被研究作为人工辅助载体细胞（aAVCs）的活性成分用于癌症治疗。细胞中α-GalCer含量的定量测定是保证aAVCs抗肿瘤作用的关键。本研究建立了一种新的液相色谱-串联质谱分析方法，采用脂质提取法和内标法，并对其进行了分析验证。此外，采用该方法测定α-GalCer加载的模型细胞（aAVCs的原始载体细胞）中α-GalCer的含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical & pharmaceutical bulletin 医学-化学综合

CiteScore

3.20

自引率

5.90%

发文量

132

审稿时长

1.7 months

期刊介绍： The CPB covers various chemical topics in the pharmaceutical and health sciences fields dealing with biologically active compounds, natural products, and medicines, while BPB deals with a wide range of biological topics in the pharmaceutical and health sciences fields including scientific research from basic to clinical studies. For details of their respective scopes, please refer to the submission topic categories below. Topics: Organic chemistry In silico science Inorganic chemistry Pharmacognosy Health statistics Forensic science Biochemistry Pharmacology Pharmaceutical care and science Medicinal chemistry Analytical chemistry Physical pharmacy Natural product chemistry Toxicology Environmental science Molecular and cellular biology Biopharmacy and pharmacokinetics Pharmaceutical education Chemical biology Physical chemistry Pharmaceutical engineering Epidemiology Hygiene Regulatory science Immunology and microbiology Clinical pharmacy Miscellaneous.