Integrated multi-omics analysis of UL16-binding protein 2 as a prognostic and immunotherapy biomarker for colorectal cancer.

Abstract

Background: UL16-binding protein 2 (ULBP2) is an important immune regulatory molecule involved in natural killer cell activation and tumor immune surveillance, although the specific role and clinical significance in colorectal cancer (CRC) require further exploration.

Methods: ULBP2 expression in CRC vs. normal tissues was analyzed using The Cancer Genome Atlas and the Gene Expression Omnibus databases, along with potential associations with clinicopathological features, while the diagnostic value was assessed with receiver operating characteristic (ROC) curves and the prognostic impact by Kaplan-Meier and Cox regression analyses. Additionally, the regulatory mechanisms of ULBP2 were explored by investigations of promoter DNA methylation, m6A regulation, and immune cell infiltration. Finally, cellular experiments were conducted to evaluate ULBP2 as a potential biomarker to predict CRC progression.

Results: ULBP2 upregulation was significantly correlated with an advanced pathological stage of CRC. ROC curve analysis indicated that ULBP2 has strong diagnostic value. Kaplan-Meier survival analysis showed that high ULBP2 expression was predictive of a poorer prognosis. Cox regression analysis highlighted ULBP2 as an independent prognostic factor. ULBP2 expression was linked to promoter methylation, m6A regulation, and immune cell infiltration. Cellular experiments showed that ULBP2 knockdown suppressed CRC progression.

Conclusion: ULBP2 has potential as a prognostic biomarker and therapeutic target of CRC. These findings provide valuable insights for future studies of tumorigenic mechanisms and clinical applications.