Separation and aggregation of extracellular vesicles by microfluidics.

IF 3.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Biomedical Microdevices Pub Date : 2025-06-23 DOI:10.1007/s10544-025-00752-3

Ziyan Zhang, Yufeng Zhou

引用次数: 0

Abstract

Membrane-bound extracellular vesicles (EVs) are more than mere messengers; they are the carriers of intercellular communication, carrying biomolecules for regulatory processes. They have potential in biomarker discovery and disease diagnosis for clinical applications. However, the exploration and utilization of EVs are currently constrained by the existing processing methodologies. Microfluidic technology is a versatile platform, achieving the efficient, consistent, and precise separation and aggregation of particles from the nanoscale to the microscale. It has great potential for EVs, enabling precise manipulation, separation, and aggregation in microchannels. This review explores active and passive microfluidic techniques, presenting a cost-effective and scalable solution for label-free separation. Their development is important for EV research, unlocking value in the in-depth study. Their innovative biomedical applications can revolutionize laboratory medicine, drug delivery, and regenerative medicine by fully realizing and harnessing the potential of EVs.

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微流体对细胞外囊泡的分离和聚集。

膜结合细胞外囊泡（EVs）不仅仅是信使；它们是细胞间通讯的载体，携带生物分子进行调节过程。它们在生物标志物发现和疾病诊断方面具有潜在的临床应用价值。然而，目前电动汽车的开发和利用受到现有加工方法的制约。微流控技术是一个多功能的平台，实现了从纳米级到微米级的粒子的高效、一致和精确的分离和聚集。它对电动汽车具有巨大的潜力，可以在微通道中实现精确的操作、分离和聚合。本综述探讨了主动和被动微流体技术，提出了一种具有成本效益和可扩展的无标签分离解决方案。它们的发展对电动汽车的研究具有重要意义，在深入研究中释放出价值。通过充分实现和利用电动汽车的潜力，它们的创新生物医学应用可以彻底改变实验室医学、药物输送和再生医学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.