Development of Differential Ion Mobility Spectrometry Based Tandem Devices for Ion Separation Prior to Mass Spectrometry Analysis

IF 1.7 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-06-24 DOI:10.1002/rcm.10099

Simin Zhang, T.-Y. Lui, Danna Hu, Xiangfeng Chen, T.-W. Dominic Chan

{"title":"Development of Differential Ion Mobility Spectrometry Based Tandem Devices for Ion Separation Prior to Mass Spectrometry Analysis","authors":"Simin Zhang, T.-Y. Lui, Danna Hu, Xiangfeng Chen, T.-W. Dominic Chan","doi":"10.1002/rcm.10099","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Rationale</h3>\n \n <p>Differential ion mobility spectrometry (DMS) provides distinct potential in separation of gas phase ions. However, the resolving power still limits the widespread application of DMS technology and inspires to design tandem DMS for improvement of the performance.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>A robust tandem DMS module was fabricated to enhance the separation performance of the DMS system. To achieve the optimal separation, two carrier gas composition modulation strategies were carried out by adding different types of organic modifiers and by changing the modifier concentration in each DMS channel. The working performance of the home-made CaptiveSpray-tandem DMS-MS was evaluated by investigating the peak intensity and FWHM using reserpine and comparing the peak positions of peptide mixtures in two DMS channels.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>When carrier gas composition in two channels was controlled appropriately, the tandem DMS system could provide the DMS system with another dimension for ion separation and therefore enhance the separation ability. Two gas modification methods could be applied in this system for enhancing analytical performance.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This tandem DMS platform allows precise control of the gap widths of the DMS channels and enables the modulation of carrier gas composition in two DMS channels separately. The developed method is capable of providing orthogonal separation behavior and achieving higher separation performance.</p>\n </section>\n </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 19","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rapid Communications in Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/rcm.10099","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Rationale

Differential ion mobility spectrometry (DMS) provides distinct potential in separation of gas phase ions. However, the resolving power still limits the widespread application of DMS technology and inspires to design tandem DMS for improvement of the performance.

Methods

A robust tandem DMS module was fabricated to enhance the separation performance of the DMS system. To achieve the optimal separation, two carrier gas composition modulation strategies were carried out by adding different types of organic modifiers and by changing the modifier concentration in each DMS channel. The working performance of the home-made CaptiveSpray-tandem DMS-MS was evaluated by investigating the peak intensity and FWHM using reserpine and comparing the peak positions of peptide mixtures in two DMS channels.

Results

When carrier gas composition in two channels was controlled appropriately, the tandem DMS system could provide the DMS system with another dimension for ion separation and therefore enhance the separation ability. Two gas modification methods could be applied in this system for enhancing analytical performance.

Conclusions

This tandem DMS platform allows precise control of the gap widths of the DMS channels and enables the modulation of carrier gas composition in two DMS channels separately. The developed method is capable of providing orthogonal separation behavior and achieving higher separation performance.

查看原文本刊更多论文

基于差分离子迁移率谱的串联装置在质谱分析之前用于离子分离

差分离子迁移谱法（DMS）在气相离子的分离中具有明显的潜力。然而，分辨率的问题仍然限制了DMS技术的广泛应用，激发了人们设计串联DMS以提高性能。方法制备鲁棒串联DMS模块，提高DMS系统的分离性能。为了实现最佳分离，通过添加不同类型的有机改性剂和改变各DMS通道中改性剂的浓度，对载气组分进行了两种调制策略。采用利血平对国产captivesray串联DMS- ms的峰强度和峰宽进行了研究，并比较了两种DMS通道中肽混合物的峰位，评价了该串联DMS的工作性能。结果在适当控制两通道载气组成的前提下，串联DMS系统可为DMS系统的离子分离提供另一个维度，从而提高DMS系统的分离能力。该系统可采用两种气体改性方法来提高分析性能。结论该串联DMS平台可以精确控制DMS通道的间隙宽度，并可以分别调制两个DMS通道中的载气成分。该方法具有正交分离特性，具有较高的分离性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.