{"title":"Integrated Microfluidic Flexible Test Strip for on-Chip Micromixing and Glucose Sensing With Smartphone-Based Chemiluminescence Platform","authors":"Kavita Manekar;Meghana A. Hasamnis","doi":"10.1109/LSENS.2025.3572024","DOIUrl":null,"url":null,"abstract":"Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an <italic>R</i><sup>2</sup> of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.","PeriodicalId":13014,"journal":{"name":"IEEE Sensors Letters","volume":"9 7","pages":"1-4"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Letters","FirstCategoryId":"1085","ListUrlMain":"https://ieeexplore.ieee.org/document/11007486/","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an R2 of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.