Eco-friendly synthesis of ZnO nanoparticles using pomegranate leaves and its antimicrobial, antioxidant, anticancer, angiogenic activity, and toxicity assessment

IF 4.2 Q2 CHEMISTRY, MULTIDISCIPLINARY
Pradnya Sunil Bapte , Shreyas Pansambal , Pranav P. Bardapurkar , Suresh Ghotekar , Seema Sachin Borgave
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Abstract

Green-synthesized nanoparticles are cost-effective, environment-friendly, and less hazardous, with potentially diverse biological applications. Nanoparticles of zinc oxide (ZnO) were generated with an aqueous extract of dried pomegranate leaves and characterized using X-ray diffractometry, Infrared and UV–visible spectroscopy, and field emission scanning electron microscopy (FESEM) tools. The synthesized ZnO nanoparticles (NPs) demonstrated comparable and enhanced antioxidant potential to the leaf extract or zinc nitrate. These ZnO nanoparticles showed antimicrobial efficacy, noticeably against C. albicans, E. faecalis, and P. aeruginosa. Though the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays indicated more significant toxicity to normal cells (HaCaT) than malignant cells (MCF-7), the recorded anticancer efficacy of our nanoparticles was more pronounced than that of other reported nanoparticles. The chorioallantoic membrane assay, deployed to assess the angiogenic potential of the nanoparticles, unveiled dose-dependent promotion or inhibition of vascular sprouting activity. The toxicological effects of the synthesized nanoparticles on the early embryos were examined utilizing Zebrafish as a model organism. The death rate of early treated embryos was found to be directly proportional to the nanoparticle doses. At low doses, the ZnO nanoparticles did not hamper the development of embryos. In contrast, the embryos that survived higher ZnO concentrations exhibited developmental defects, primarily in hatching rate, eye pigmentation, and tail deformities, which were monitored until 72 h post-fertilization. The ZnO nanoparticles synthesized in the present study exhibited remarkable antioxidant, anticancer, and anti-angiogenic potentials, which warrant further thorough investigations into their potential applications in medicine.

Abstract Image

石榴叶环保合成ZnO纳米粒子及其抗菌、抗氧化、抗癌、血管生成活性和毒性评价
绿色合成的纳米颗粒具有成本效益高、环境友好、危害小的特点,具有潜在的多种生物应用。以石榴干叶水提物为原料制备氧化锌纳米颗粒,并利用x射线衍射、红外和紫外可见光谱以及场发射扫描电子显微镜(FESEM)对其进行了表征。合成的ZnO纳米颗粒(NPs)表现出与叶提取物或硝酸锌相当的抗氧化能力。氧化锌纳米颗粒对白色念珠菌、粪肠杆菌和铜绿假单胞菌具有明显的抗菌作用。虽然MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑)试验表明对正常细胞(HaCaT)的毒性比对恶性细胞(MCF-7)的毒性更显著,但我们的纳米颗粒的抗癌功效比其他报道的纳米颗粒更明显。绒毛膜尿囊膜试验用于评估纳米颗粒的血管生成潜力,揭示了剂量依赖性的血管发芽活性促进或抑制。以斑马鱼为模型生物,研究了合成纳米颗粒对早期胚胎的毒理学影响。研究发现,早期处理胚胎的死亡率与纳米颗粒剂量成正比。在低剂量下,ZnO纳米颗粒不会阻碍胚胎的发育。相比之下,在高氧化锌浓度下存活下来的胚胎表现出发育缺陷,主要表现在孵化率、眼睛色素沉着和尾巴畸形上,这些缺陷一直持续到受精后72 h。本研究合成的氧化锌纳米颗粒具有显著的抗氧化、抗癌和抗血管生成潜能,值得进一步深入研究其在医学上的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Results in Chemistry
Results in Chemistry Chemistry-Chemistry (all)
CiteScore
2.70
自引率
8.70%
发文量
380
审稿时长
56 days
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