Diet and environment drive the convergence of gut microbiome in wild-released giant pandas and forest musk deer

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-06-07 DOI:10.1016/j.isci.2025.112837

Chenyi Gao , Qiuyu Huang , Xinru Yang , Xinyuan Cui , Kaizhi Wen , Yue Liu , Chenyan Wang , Qinlong Dai , Jiadong Xie , Lifeng Zhu

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Abstract

Reintroduction is important for recovering endangered species, and gut microbiome is crucial for successful wildlife reintroduction. This study utilized 16S rRNA high-throughput sequencing of 791 fecal samples to examine the gut microbial changes in giant pandas (Ailuropoda melanoleuca) and forest musk deer (Moschus berezovskii) across captivity, semi-release, and release stages. Our results revealed a similar transitional pattern in the gut microbiome of both species, with semi-release stage displaying an intermediate state between captive and wild microbiome. We also observed that both species are enriched in Pseudomonas and functional pathways related to amino acid metabolism, ATP-binding cassette transporters, and acetyl-CoA/propionyl-CoA carboxylase. Furthermore, the SourceTracker analysis indicated putative contributions of plant and soil microbiome to the gut microbiome of forest musk deer. These findings suggest that similar herbivorous diets and same environment may contribute to the convergence of gut microbiome. In conclusion, our study provides valuable insights for reintroducing endangered wildlife.

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野生大熊猫和森林麝的饮食和环境驱动肠道微生物群的趋同

重新引入对濒危物种的恢复至关重要，而肠道微生物群对野生动物的成功重新引入至关重要。本研究利用16S rRNA高通量测序技术对791份粪便样本进行了研究，分析了大熊猫（Ailuropoda melanoleuca）和森林麝（Moschus berezovskii）在圈养、半放养和放养阶段肠道微生物的变化。我们的研究结果揭示了两种物种肠道微生物群的过渡模式相似，半释放阶段显示了圈养和野生微生物群之间的中间状态。我们还观察到，这两个物种都富含假单胞菌和氨基酸代谢、atp结合盒转运蛋白和乙酰辅酶a /丙酰辅酶a羧化酶相关的功能途径。此外，SourceTracker分析显示了植物和土壤微生物组对森林麝肠道微生物组的推定贡献。这些发现表明，相似的草食性饮食和相同的环境可能有助于肠道微生物群的趋同。总之，我们的研究为重新引入濒危野生动物提供了有价值的见解。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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