Enteric neural crest development in Astyanax mexicanus surface fish and cavefish

IF 2.6 3区生物学 Q4 CELL BIOLOGY

Differentiation Pub Date : 2025-06-13 DOI:10.1016/j.diff.2025.100881

Pavani Ponnimbaduge Perera , Kaitlyn Webster , Misty R. Riddle

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引用次数: 0

Abstract

The enteric nervous system (ENS) regulates gastrointestinal (GI) functions such as secretion, blood flow, and motility, yet how its structure and function evolve with dietary adaptations remains unclear. Astyanax mexicanus, a teleost fish with surface and cave morphotypes, offers a model to explore these changes; cavefish exhibit altered GI motility and transit that may help them adapt to their unique diet. We compared early ENS development in surface fish and cavefish, tracking enteric neural crest cell (ENCC) migration and differentiation using phox2bb and HuC/D markers. We found that ENCCs reach the gut by 36 hours post-fertilization (hpf) in both morphotypes but migrate and differentiate more rapidly along the gut in cavefish. To explore the genetic basis of this difference, we used available genomic datasets to compare the predicted peptide sequences of genes important for ENS development in other species and identified mutations that could impact protein function, for example in the endothelin signaling genes important for ENCC migration and differentiation. We specifically examined the expression of endothelin-3 (edn3) and endothelin receptor-b a (ednrba) during ENCC migration and found that the localization of edn3, but not ednrba, is consistent with a potential role in ENS development. Overall, our findings establish A. mexicanus as a model for studying evolution of ENS development.

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面鱼和穴居鱼肠道神经嵴发育的研究

肠神经系统（ENS）调节胃肠道（GI）功能，如分泌、血流和运动，但其结构和功能如何随着饮食适应而进化尚不清楚。Astyanax mexicanus，一种具有表面和洞穴形态的硬骨鱼，为探索这些变化提供了一个模型；洞穴鱼表现出改变的胃肠道运动和运输，这可能有助于它们适应独特的饮食。我们比较了表层鱼和洞穴鱼的早期ENS发育，利用phox2bb和HuC/D标记跟踪了肠神经嵴细胞（enteric neural crest cell， ENCC）的迁移和分化。我们发现，两种形态的ENCCs在受精后36小时到达肠道，但在洞穴鱼中，ENCCs沿着肠道迁移和分化的速度更快。为了探索这种差异的遗传基础，我们使用现有的基因组数据集来比较其他物种ENS发育重要基因的预测肽序列，并确定可能影响蛋白质功能的突变，例如对ENCC迁移和分化重要的内皮素信号基因。我们专门检查了内皮素-3 （edn3）和内皮素受体-b - a （ednrba）在ENCC迁移过程中的表达，发现edn3的定位，而不是ednrba，与ENS发展的潜在作用一致。总的来说，我们的研究结果建立了墨西哥拟南猿作为研究ENS发展进化的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Differentiation 生物-发育生物学

CiteScore

4.10

自引率

3.40%

发文量

审稿时长

51 days

期刊介绍： Differentiation is a multidisciplinary journal dealing with topics relating to cell differentiation, development, cellular structure and function, and cancer. Differentiation of eukaryotes at the molecular level and the use of transgenic and targeted mutagenesis approaches to problems of differentiation are of particular interest to the journal. The journal will publish full-length articles containing original work in any of these areas. We will also publish reviews and commentaries on topics of current interest. The principal subject areas the journal covers are: • embryonic patterning and organogenesis • human development and congenital malformation • mechanisms of cell lineage commitment • tissue homeostasis and oncogenic transformation • establishment of cellular polarity • stem cell differentiation • cell reprogramming mechanisms • stability of the differentiated state • cell and tissue interactions in vivo and in vitro • signal transduction pathways in development and differentiation • carcinogenesis and cancer • mechanisms involved in cell growth and division especially relating to cancer • differentiation in regeneration and ageing • therapeutic applications of differentiation processes.