Icariin mitigates experimental contrast-induced nephropathy: Mechanistic insights

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-06-18 DOI:10.1016/j.tice.2025.103020

Hanan A. Elgendy , Amira Osman , Amira E. Farage , Medhat Taha , Sara Abubakr , Alaa.M. Badawy , Mohie Mahmoud Ibrahim , Ahmed Nabawy Ahmed Nasr , Hala Mahfouz , Tourki A.S. Baokbah , Ahmed shaban Abdelmonsef , Mohamed Ezzat Mahmoud , Ahmed Abdel-monem Elmetwally , Abdulrahman I. AbuAqil , Nora Elshehawy Helal

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引用次数: 0

Abstract

Background

Currently, the use of contrast agents in interventional procedures and imaging has been increasing. Icariin, an active component of Epimedium, offers beneficial biological activities such as antioxidation and anti-inflammation. Therefore, our study aimed to investigate the protective role of icariin and its underlying mechanisms against contrast-induced nephropathy (CIN).

Methods

We divided twenty-four rats into four groups (n = 6 each): control group icariin-only treated group, contrast-induced nephropathy (CIN) group, and CIN+Icariin group, with six rats in each group. We performed renal function tests (serum creatinine and blood urea nitrogen (BUN) and histological evaluations. Additionally, we measured malondialdehyde (MDA) and superoxide dismutase (SOD) levels in renal supernatant and used ELISA to quantify caspase-8, caspase-9, cytochrome c, Bcl-2 associated protein x (Bax), and B cell lymphoma/leukemia 2 protein (Bcl-2) levels. We assessed nuclear factor kappa beta (NF-κB) and interleukin-1 beta (IL-1β) gene expression and evaluated immunoexpression of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), CD68, and caspase-3.

Results

Icariin showed nephroprotective effects against CIN, demonstrated by reduced creatinine and BUN; decreased MDA, IL-6, NF-κB, TNF-α, IL-1β, CD68, caspase-8, caspase-9, Bax, and cytochrome c; and increased SOD and Bcl-2 levels. Renal histology improved in the CIN+Icariin group compared to CIN group.

Conclusions

Oral administration of 100 mg/kg icariin exhibited renoprotective effects against contrast-induced renal injury through anti-inflammatory, anti-apoptotic, and antioxidant mechanisms.

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淫羊藿苷减轻实验性造影剂肾病：机制见解

目前，造影剂在介入手术和影像学中的应用越来越多。淫羊藿的活性成分淫羊藿苷具有抗氧化、抗炎等生物活性。因此，我们的研究旨在探讨淫羊藿苷对造影剂肾病（CIN）的保护作用及其潜在机制。方法将24只大鼠分为4组（n = 6只）：对照组、造影剂肾病（CIN）组、CIN+Icariin组，每组6只。我们进行了肾功能检查（血清肌酐和血尿素氮（BUN））和组织学评估。此外，我们测量了肾脏上清中丙二醛（MDA）和超氧化物歧化酶（SOD）的水平，并使用ELISA法定量caspase-8、caspase-9、细胞色素c、Bcl-2相关蛋白x （Bax）和B细胞淋巴瘤/白血病2蛋白（Bcl-2）的水平。我们评估核因子κ b （NF-κB）和白细胞介素-1β (IL-1β)基因表达，并评估肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）、CD68和caspase-3的免疫表达。结果淫羊藿苷对CIN具有肾保护作用，表现为降低肌酐和BUN；降低MDA、IL-6、NF-κB、TNF-α、IL-1β、CD68、caspase-8、caspase-9、Bax、细胞色素c；SOD和Bcl-2水平升高。与CIN组相比，CIN+淫羊藿苷组肾脏组织学改善。结论100 mg/kg淫羊藿苷通过抗炎、抗凋亡和抗氧化等机制对造影剂所致肾损伤具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.