Dual-mode colorimetric-SPR immunosensor based on gold nanoparticles for sCD146 detection in plasma

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-06-21 DOI:10.1016/j.bios.2025.117702

Zhaochen Huo , Qilong Li , Miaomiao Liu , Xiaofei Xing , Xiangheng Fan , Yilin Wang

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引用次数: 0

Abstract

Soluble cluster of differentiation 146 (sCD146) is expressed in various immune cells and plays crucial roles in cellular processes linked to tumor cell metastasis, as well as in cell signalling, migration, proliferation, and inflammation. Detecting sCD146 in plasma has become a promising approach for early cancer screening. A rapid and highly specific dual-mode immunosensor that integrates colorimetry and surface plasmon resonance (SPR) techniques was successfully developed in this study. Specifically, bovine serum albumin (BSA)-modified gold nanoparticles (AuNPs) with exceptional peroxidase (POD) activity and SPR properties were synthesized. Under optimum conditions, the linear range of the immunosensors for sCD146 detection was 5–600 ng/mL. The detection limits (LODs) were 4 ng/mL (colorimetric) and 1 ng/mL (SPR). In addition, the results of this colorimetric and SPR dual-mode immunosensor were essentially consistent with those of the conventional ELISA method (P > 0.05). This dual-mode immunosensor based on AuNPs not only has significant potential for detecting sCD146 in plasma but also offers a versatile and efficient platform for blood biomarker analysis. This novel technology opens new avenues for diagnosing cancer and predicting cancer prognosis, thereby having substantial implications for both scientific research and clinical applications.

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基于金纳米粒子的双模比色- spr免疫传感器检测血浆中sCD146

可溶性分化簇146 （sCD146）在多种免疫细胞中表达，在与肿瘤细胞转移、细胞信号传导、迁移、增殖和炎症相关的细胞过程中发挥关键作用。在血浆中检测sCD146已成为早期癌症筛查的有希望的方法。本研究成功开发了一种集成比色法和表面等离子体共振（SPR）技术的快速、高特异性双模免疫传感器。具体而言，合成了具有特殊过氧化物酶活性和SPR性能的牛血清白蛋白修饰金纳米颗粒（AuNPs）。在最佳条件下，免疫传感器检测sCD146的线性范围为5 ~ 600 ng/mL。检出限分别为4 ng/mL（比色法）和1 ng/mL （SPR法）。此外，该比色和SPR双模免疫传感器的结果与传统ELISA方法的结果基本一致(P >；0.05)。这种基于AuNPs的双模免疫传感器不仅具有检测血浆中sCD146的巨大潜力，而且为血液生物标志物分析提供了一个多功能和高效的平台。这项新技术为癌症诊断和预测癌症预后开辟了新的途径，从而对科学研究和临床应用具有重大意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.