Oral inoculation of turkeys with in vitro cultured Histomonas meleagridis in cecal contents results in histomonosis

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Applied Poultry Research Pub Date : 2025-06-06 DOI:10.1016/j.japr.2025.100560

Catherine Fudge , Olivia Wedegaertner , Katherine Cupo , Justin Lowery , Lin Walker , Frank Edens , Chongxiao Chen

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引用次数: 0

Abstract

This study examined the effect of oral administration of Histomonas meleagridis on infection rate and disease progression of histomonosis in turkeys. Experiment 1, poults were placed in 3 experimental groups consisting of H. meleagridis culture in media administered via intracloacal inoculation (CIM), oral inoculation (OIM), or oral inoculation mixed with previously frozen cecal content (OICC). Poults were inoculated with 50,000 histomonads at 8 AM and 5 PM for five days starting from 14-day of age. Cloacal inoculation (CIM) produced 100 % infection rates, while oral infection with H. meleagridis in media (OIM) led to 8 % infection. However, oral inoculations using cecal content (OICC) produced 43 % infection rates (P = 0.0003). In experiment two, poults were placed in 2 experimental groups consisting oral inoculations with fresh cecal contents containing H. meleagridis (OI) and intracloacal inoculations with fresh cecal contents containing H. meleagridis (CI). Poults were inoculated with 50,000 histomonads at 8 AM and 5 PM for five days starting from 14-day of age. Oral inoculations (OI) produced 90 % infection rates, similar to the cloacal route (CI) at 93 % (P = 0.6433). In Experiment 3, individual isolates of H. meleagridis in cecal content were administered orally: Buford isolate (BUF), Arkansas isolate (ARK), or Zeeland, Michigan isolate (ZMI). Results showed BUF and ARK isolates produced higher infection rates than the ZMI isolate (P = 0.0136). In summary, oral infection in turkeys with H. meleagridis could be achieved using cecal content and infection rates and disease progression are impacted by isolate and inoculum condition.

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用体外培养的肉鸡组织单胞菌在火鸡盲肠内容物中口服接种可导致组织单胞菌病

本研究考察了口服组织单胞菌对火鸡组织病感染率和疾病进展的影响。实验1：将肉鸡分别分为3个试验组，分别在局部接种（CIM）、口服接种（OIM）或混合冷冻盲肠内容物（OICC）的培养基中培养肉鸡弧菌。从14日龄开始，分别于上午8点和下午5点接种5万个组织单胞菌，连续5天。阴囊接种（CIM）的感染率为100%，而口腔感染培养基（OIM）的感染率为8%。然而，使用盲肠内容物（OICC）进行口服接种的感染率为43% （P = 0.0003）。试验二将雏鸡分为2个试验组，分别为新鲜盲肠内容物含肉鸡肉鸡（OI）和新鲜盲肠内容物含肉鸡肉鸡肉鸡（CI）的口腔接种组。从14日龄开始，分别于上午8点和下午5点接种5万个组织单胞菌，连续5天。口服接种（OI）的感染率为90%，与肠腔途径（CI）相似，为93% （P = 0.6433）。在实验3中，在盲肠内容物中，分别口服布福德分离株（BUF）、阿肯色州分离株（ARK）或密歇根州泽兰分离株（ZMI）。结果BUF和ARK分离株的感染率高于ZMI分离株（P = 0.0136）。综上所述，火鸡的口腔感染可以通过盲肠内容物来实现，感染率和疾病进展受分离和接种条件的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Poultry Research 农林科学-奶制品与动物科学

CiteScore

4.10

自引率

10.50%

发文量

审稿时长

104 days

期刊介绍： The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.