Maternal exposure to difenoconazole-induced developmental defects and its mechanisms in F1 zebrafish (Danio rerio)

IF 6.1 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Ying Zhang , Fuqi Yang , Qian Zhang , Yunan Wu , Jiaolong Huang , Peng Duan , Xingjian Zhou
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引用次数: 0

Abstract

Difenoconazole (DFZ), a representative triazole fungicide, has become ubiquitously detectable in aquatic systems owing to its extensive agricultural utilization, potentially jeopardizing ecological health through bioaccumulation. Despite its environmental prevalence, intergenerational consequences following maternal DFZ exposure remain poorly characterized. Herein, we systematically examined F1 offspring developmental impacts by exposing maternal zebrafish to environmentally realistic DFZ concentrations (3, 30 and 300 μg/L) for 28 days (d). Comprehensive phenotypical and molecular assessments were subsequently performed on F1 progeny, incorporating physiological biomarkers evaluation, histological examination, transcriptomic profiling, and quantitative validation of dysregulated signaling pathways. Maternal DFZ exposure elicited multidimensional developmental impairments in F1 larvae, characterized by morphological anomalies including pericardial/yolk sac edema, diminished cardiac contraction frequency, as well as shortened body axis and reduced ocular dimensions. Furthermore, neurosensory dysfunction manifested through attenuated spontaneous motility and compromised motor coordination capacities. Transcriptomic profiling identified organ-specific transcriptional dysregulation: cardiac development (tbx5, nkx2.5, myl7, myh6 and vmhc), eye development (rhol, opn1mw4, opn1sw1 and opn1sw2), and neurodevelopment (ache, syn2a, manf, gap43, gfap, gpx4a and mbp) were abnormal in F1 larvae. RNA-Seq showed that the cardiac muscle contraction, heart morphogenesis, eye development, neuron differentiation, GABAergic synapse, skeletal system development and osteoclast differentiation signalling pathways were significantly enriched. Taken together, our results demonstrated that maternal zebrafish DFZ exposure at environmentally relevant concentrations potentially resulted in growth retardation and developmental disorders in F1 generation, providing new insight into the underlying maternal transfer effects.
母体暴露于异丙康唑诱导的F1斑马鱼发育缺陷及其机制
二苯醚康唑(Difenoconazole, DFZ)是一种具有代表性的三唑类杀菌剂,由于其广泛的农业利用,在水生系统中已无处不在,可能通过生物积累危害生态健康。尽管其在环境中普遍存在,但母体DFZ暴露后的代际后果仍不清楚。在此,我们通过将母斑马鱼暴露在环境现实的DFZ浓度(3,30和300 μg/L)中28天(d),系统地研究了F1后代的发育影响。随后对F1后代进行了全面的表型和分子评估,包括生理生物标志物评估、组织学检查、转录组学分析和失调信号通路的定量验证。母体DFZ暴露会引起F1幼虫的多维发育障碍,其特征是形态学异常,包括心包/卵黄囊水肿、心脏收缩频率降低、体轴缩短和眼尺寸减小。此外,神经感觉功能障碍表现为自发性运动减弱和运动协调能力受损。转录组学分析发现了器官特异性转录失调:F1幼虫的心脏发育(tbx5、nkx2.5、my17、myh6和vmhc)、眼睛发育(rhol、opn1mw4、opn1sw1和opn1sw2)和神经发育(ache、syn2a、manf、gap43、gfap、gpx4a和mbp)异常。RNA-Seq显示心肌收缩、心脏形态发生、眼睛发育、神经元分化、gaba能突触、骨骼系统发育和破骨细胞分化信号通路显著富集。综上所述,我们的研究结果表明,在环境相关浓度下,母体斑马鱼DFZ暴露可能导致F1代生长迟缓和发育障碍,为潜在的母体转移效应提供了新的见解。
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来源期刊
CiteScore
12.10
自引率
5.90%
发文量
1234
审稿时长
88 days
期刊介绍: Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.
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