Orientation-independent bubble trap with internal partition for robust operation of microfluidic systems†

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-06-23 DOI:10.1039/D4LC01060D
Bhavagyna Vegunta, Charmaine Lui, Max Kim, Joshua Tran, Maya Papez, Anand K. Ramasubramanian and Sang-Joon John Lee
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Abstract

A new monolithic bubble trap has been developed with a unique, orientation-independent design. The bubble trap has a spherical cavity and a central partition with internal passages that eliminate air bubbles effectively for extended periods of time. Flow testing was performed in a closed-loop microfluidic system to demonstrate effectiveness and robustness of the bubble trap. Flow rate was continually monitored on a stationary benchtop and also in simulated microgravity conditions on a 3-D random positioning machine. Data collected from flow sensors placed fore and aft of the bubble trap confirmed that randomly occurring bubbles were effectively eliminated by the trap throughout 24 hour closed-loop perfusion tests. To highlight the orientation-independent benefit of the bubble trap in a specific application of interest, continuous-flow experiments were conducted using human umbilical vein endothelial cells in a closed-loop microfluidic system. The bubble trap successfully protected the integrity of confluent layers that otherwise suffered from cell detachment without the trap. Image analysis showed that random orientation reduced directional alignment of cell nuclei, relative to baseline experiments performed under normal gravity.

Abstract Image

Abstract Image

用于微流体系统鲁棒运行的具有内部隔板的非定向气泡阱
一种新的单片气泡阱具有独特的、与方向无关的设计。气泡捕集器具有球形腔和具有内部通道的中央隔板,该内部通道可在延长的时间内有效地消除气泡。在闭环微流体系统中进行了流量测试,以验证气泡阱的有效性和鲁棒性。在固定的工作台上和模拟微重力条件下的三维随机定位机上连续监测流量。从放置在气泡捕集器前后的流量传感器收集的数据证实,在24小时闭环灌注试验中,该捕集器有效地消除了随机出现的气泡。为了突出气泡陷阱在特定应用中的定向无关性优势,在闭环微流体系统中使用人脐静脉内皮细胞进行了连续流实验。气泡陷阱成功地保护了融合层的完整性,否则没有陷阱就会导致细胞脱离。图像分析显示,相对于在正常重力下进行的基线实验,随机定向降低了细胞核的定向排列。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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