Multi-activity of Nanozyme Liposome Regulates the Homeostasis of Biochemical Microenvironment

Abstract

In living systems, cascade reactions involving multiple enzymes, critical for regulating the biochemical microenvironment’s homeostasis, are essential to transplantation remodeling. However, for ready-to-use purposes, designing and constructing therapeutic liposomes that replicate natural cell functions during the initial transplantation phase remains a significant challenge in synthetic biology for tissue engineering. Herein, we developed a biomimetic liposome with multi-enzymatic nanozyme activity to regulate proinflammatory factors induced by exogenous implants. The loposomes were fabricated through the self-assembly of artificial membrane on CeO2 nanoparticles (NPs). These membranes, featuring a negatively charged surface analogous to endothelial cell (EC) membranes, effectively attenuate blood component adhesion and aggregation. The CeO2 nanozyme not only hydrolyzes adenosine triphosphate/adenosine diphosphate (ATP/ADP) to adenosine Monophosphate (AMP), reducing subsequent platelet aggregation, but also regulates biochemical microenvironment homeostasis, thereby promoting tissue regeneration. This work advances the development of cell-like entities capable of modulating signaling communication between living and abiotic interfaces.