Transcriptomic analysis of theca cells in a PCOS rat model: insights into follicular development.

IF 3.8 3区医学 Q1 REPRODUCTIVE BIOLOGY

Journal of Ovarian Research Pub Date : 2025-06-21 DOI:10.1186/s13048-025-01663-w

Chen Yuanyuan, Sun Ningyu, Lu Lu, Zhang Wuwen, Li Kai, Li Yanting, He Junlan, Cheng Xiang, Zhou Jing, Yan Hua, Yin Ping

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引用次数: 0

Abstract

Background: This study aimed to analyze differentially expressed genes in theca cells of polycystic ovary syndrome (PCOS) rats using transcriptomic sequencing. Bioinformatics analysis and PCR validation were performed to identify genes involved in follicular development regulation in PCOS.

Methods: Twenty 6-week-old female SD rats with regular estrous cycles were divided into two groups (PCOS and control, n = 10 each). The PCOS model was induced with a 1.0 mg·kg⁻¹ Letrozole solution. Theca cells were collected for transcriptomic sequencing, and differentially expressed genes were analyzed. Functional annotations and pathway enrichment were determined using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Gene interaction network and hub gene analyses were conducted, followed by RT-qPCR validation.

Results: PCOS rats exhibited increased body weight and irregular estrous cycles. A total of 1,114 differentially expressed genes were identified, including 516 upregulated and 598 downregulated genes. Fifty hub genes were selected for further analysis. GO and KEGG pathway enrichment analysis revealed significant involvement of the MAPK and PI3K-Akt signaling pathways. PCR validation confirmed that Cyp17a1, Cyp11a1, S6k1, mTOR, Akt, Kit, and Tek were significantly upregulated in the PCOS group (P < 0.05).

Conclusion: Transcriptomic analysis identified key genes and pathways involved in follicular development dysregulation in PCOS rats, providing potential targets for further research.

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多囊卵巢综合征大鼠模型中卵泡膜细胞的转录组学分析：对卵泡发育的见解。

背景：本研究旨在利用转录组测序技术分析多囊卵巢综合征（PCOS）大鼠卵泡膜细胞的差异表达基因。通过生物信息学分析和PCR验证，鉴定多囊卵巢综合征（PCOS）患者卵泡发育调控相关基因。方法：将20只6周龄、月经周期正常的SD雌性大鼠分为PCOS组和对照组，每组10只。用1.0 mg·kg来曲唑溶液建立PCOS模型。收集caa细胞进行转录组测序，分析差异表达基因。使用基因本体（GO）和京都基因与基因组百科全书（KEGG）分析确定功能注释和途径富集。进行基因互作网络和枢纽基因分析，并进行RT-qPCR验证。结果：多囊卵巢综合征大鼠表现为体重增加，月经周期不规则。共鉴定出1114个差异表达基因，其中上调基因516个，下调基因598个。选择50个枢纽基因进行进一步分析。GO和KEGG通路富集分析显示MAPK和PI3K-Akt信号通路显著参与。PCR验证Cyp17a1、Cyp11a1、S6k1、mTOR、Akt、Kit和Tek在PCOS组中显著上调(P)。结论：转录组学分析确定了PCOS大鼠卵泡发育异常的关键基因和通路，为进一步研究提供了潜在的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Ovarian Research REPRODUCTIVE BIOLOGY-

CiteScore

6.20

自引率

2.50%

发文量

125

审稿时长

>12 weeks

期刊介绍： Journal of Ovarian Research is an open access, peer reviewed, online journal that aims to provide a forum for high-quality basic and clinical research on ovarian function, abnormalities, and cancer. The journal focuses on research that provides new insights into ovarian functions as well as prevention and treatment of diseases afflicting the organ. Topical areas include, but are not restricted to: Ovary development, hormone secretion and regulation Follicle growth and ovulation Infertility and Polycystic ovarian syndrome Regulation of pituitary and other biological functions by ovarian hormones Ovarian cancer, its prevention, diagnosis and treatment Drug development and screening Role of stem cells in ovary development and function.