Expression of growth factors in buffalo ovarian tissue across different follicular developmental stages.

IF 2.5 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Archives of Gynecology and Obstetrics Pub Date : 2025-06-21 DOI:10.1007/s00404-025-08090-8

Seham Samir Soliman, Marwa El-Sheikh, Dalia A Taha, Karima A Hamed, Wagdy K B Khalil

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引用次数: 0

Abstract

Background: In assisted reproduction, poor ovarian response to stimulation negatively affects oocyte yield and is influenced by genetic factors.

Objective: This study aimed to quantify the mRNA expression of key growth markers (BMP15, GDF9, OCT4, and FGFR2) in ovarian tissue according to the developmental stages of the follicle.

Methods: Samples were collected from ovarian tissue. Gene expression levels were analyzed using RT-qPCR. In addition, ELISA was used to measure the concentrations of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), along with reactive oxygen species (ROS) and malondialdehyde (MDA) as oxidative stress markers.

Results: OCT4 expression was similar in preantral and small follicles but significantly upregulated in medium and large follicles. GDF9 expression and SOD activity were highest in small follicles (P < 0.05). BMP15 levels were significantly elevated in small and medium follicles compared to preantral follicles but remained unchanged in large follicles (P < 0.05). FGFR2 expression increased progressively with follicle size (P < 0.05). GPx activity was directly proportional to follicle size, with the lowest levels in preantral follicles. Conversely, ROS, MDA, and CAT concentrations decreased as follicle size increased.

Conclusion: These findings provide insights into the molecular regulation of follicular development in buffalo, which could aid in improving reproductive efficiency in assisted reproduction programs.

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不同卵泡发育阶段水牛卵巢组织中生长因子的表达。

背景：在辅助生殖中，卵巢对刺激的不良反应会影响卵母细胞的产量，并受遗传因素的影响。目的：本研究旨在根据卵泡发育阶段，定量测定卵巢组织中关键生长标志物（BMP15、GDF9、OCT4、FGFR2） mRNA的表达。方法：卵巢组织标本采集。采用RT-qPCR分析基因表达水平。此外，ELISA法检测过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的浓度，以及活性氧（ROS）和丙二醛（MDA）作为氧化应激标志物。结果：OCT4在腔前和小卵泡中表达相似，而在中、大卵泡中表达明显上调。结论：这些发现为水牛卵泡发育的分子调控提供了新的思路，有助于提高辅助生殖系统的生殖效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Gynecology and Obstetrics 医学-妇产科学

CiteScore

4.70

自引率

15.40%

发文量

493

审稿时长

1 months

期刊介绍： Founded in 1870 as "Archiv für Gynaekologie", Archives of Gynecology and Obstetrics has a long and outstanding tradition. Since 1922 the journal has been the Organ of the Deutsche Gesellschaft für Gynäkologie und Geburtshilfe. "The Archives of Gynecology and Obstetrics" is circulated in over 40 countries world wide and is indexed in "PubMed/Medline" and "Science Citation Index Expanded/Journal Citation Report". The journal publishes invited and submitted reviews; peer-reviewed original articles about clinical topics and basic research as well as news and views and guidelines and position statements from all sub-specialties in gynecology and obstetrics.