Lang Xiao , Mingzhu Ye , Yirui Fan , Guangyu Liu , Huixia He , Jianxi Xiao
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引用次数: 0
Abstract
Digital light processing (DLP) bioprinting, known for its speed and precision, has become a key tool in disease modeling and regenerative medicine. Yet, creating bioinks with optimal printability, bioactivity, and cell-supporting capacity remains a major challenge. Here, we introduce a novel dual-network collagen-based bioink, methyl acrylated collagen-dimethylphenylphosphonate (CMA-DPPA), which enables the fabrication of mechanically robust and highly printable cell-laden constructs through DLP 3D bioprinting. The CMA-DPPA hydrogel is synthesized by cross-linking collagen with DPPA, followed by photo-crosslinking with CMA; this dual-cross-linking approach markedly improves printing fidelity, mechanical strength, and enzymatic degradation resistance of the 3D-printed constructs. The CMA-DPPA hydrogel exhibits excellent biocompatibility, effectively promoting cell adhesion, proliferation, and migration. In a rat model of full-thickness skin defects, CMA-DPPA hydrogel facilitated organized collagen fiber deposition and accelerated epidermal regeneration, thereby expediting wound closure. CMA-DPPA bioink enables precise, robust scaffold fabrication, showing great promise for DLP-based tissue engineering and regenerative medicine.
期刊介绍:
Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine.
Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.