Near-Infrared Organic Room Temperature Phosphorescent Probes Targeting Fibroblast Activation Protein for Surgical Navigation of Liver Cancer

Abstract

Hepatectomy is a critical treatment for liver cancer, but achieving complete tumor removal remains challenging. The development of tumor-targeting probes capable of accurately identifying tumor locations and providing real-time intraoperative navigation is of significant clinical importance. We synthesize a tumor-targeted probe by conjugating a fibroblast activation protein inhibitor (FAPI) with near-infrared organic room temperature phosphorescence (RTP) material. We then analyze its surgical navigation performance in the liver cancer model and its imaging performance in fresh patient-derived samples. In vivo validation reveals that the probe exhibits optimal phosphorescence intensity within 48 h (8.53 × 10⁵ p s⁻¹ cm⁻² sr⁻¹) and an average signal-to-noise ratio of 16. The probe could effectively identify FAP-positive samples from liver cancer patients. The successful application of phosphorescence-guided surgery suggests that the near-infrared RTP probe holds significant clinical translational value and could serve as an auxiliary tool for the surgical treatment of liver cancer.