Ultrastructural and functional recovery of mitochondria and improved developmental competence by melatonin in oxidatively stressed porcine oocytes

IF 3.9 3区生物学 Q2 CELL BIOLOGY

Mitochondrion Pub Date : 2025-06-19 DOI:10.1016/j.mito.2025.102060

Heyyoung Kim , Seonggyu Bang , Ayeong Han , Heejae Kang , Islam M. Saadeldin , Ahmad Yar Qamar , Sanghoon Lee , Jongki Cho

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引用次数: 0

Abstract

Mitochondrial dysfunction induced by oxidative stress impairs oocyte maturation and subsequent embryonic development. In this study, we investigated whether melatonin, a potent antioxidant, could mitigate hydrogen peroxide (H₂O₂)-induced mitochondrial damage in porcine oocytes and restore their developmental competence. Oocytes were exposed to H₂O₂ prior to in vitro maturation (IVM), followed by treatment with varying concentrations of melatonin (0, 0.5, 1, and 5 μM). Melatonin treatment significantly improved maturation and blastocyst formation rates, with 1 μM showing the most pronounced effect. This recovery was accompanied by enhanced mitochondrial bioenergetics, which was likely driven by reduced ROS accumulation and increased intracellular glutathione. Melatonin also reversed the ultrastructural abnormalities of mitochondria, reduced apoptotic signals, and normalized mitophagy markers. These findings suggest that melatonin confers mitochondrial protection and promotes oocyte competence under oxidative stress, supporting its therapeutic potential in reproductive biotechnology.

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褪黑素对氧化应激猪卵母细胞线粒体超微结构和功能的恢复及发育能力的改善

氧化应激诱导的线粒体功能障碍损害卵母细胞成熟和随后的胚胎发育。在这项研究中，我们研究了褪黑激素（一种有效的抗氧化剂）是否可以减轻过氧化氢（H2O2）诱导的猪卵母细胞线粒体损伤，并恢复其发育能力。卵母细胞在体外成熟（IVM）之前暴露于H2O2中，然后用不同浓度的褪黑激素（0、0.5、1和5 μM）处理。褪黑素治疗显著提高了成熟和囊胚形成率，其中以1 μM效果最显著。这种恢复伴随着线粒体生物能量的增强，这可能是由ROS积累减少和细胞内谷胱甘肽增加所驱动的。褪黑素还能逆转线粒体超微结构异常，减少凋亡信号，并使线粒体自噬标记物正常化。这些发现表明，褪黑激素赋予线粒体保护并促进氧化应激下的卵母细胞能力，支持其在生殖生物技术中的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mitochondrion 生物-细胞生物学

CiteScore

9.40

自引率

4.50%

发文量

审稿时长

13.6 weeks

期刊介绍： Mitochondrion is a definitive, high profile, peer-reviewed international research journal. The scope of Mitochondrion is broad, reporting on basic science of mitochondria from all organisms and from basic research to pathology and clinical aspects of mitochondrial diseases. The journal welcomes original contributions from investigators working in diverse sub-disciplines such as evolution, biophysics, biochemistry, molecular and cell biology, genetics, pharmacology, toxicology, forensic science, programmed cell death, aging, cancer and clinical features of mitochondrial diseases.