Suppression of TaHDA8-mediated lysine deacetylation of TaAREB3 acts as a drought adaptive mechanism in wheat root development

IF 17.1 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Plant Pub Date : 2025-06-21 DOI:10.1016/j.molp.2025.06.012

Zehui Liu, Qun Yang, Xingbei Liu, Jinpeng Li, Lei Zhang, Wei Chu, Jingchen Lin, Debiao Liu, Danyang Zhao, Xiao Peng, Chaowu Zeng, Mingming Xin, Yingyin Yao, Huiru Peng, Zhongfu Ni, Qixin Sun, Zhaorong Hu

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引用次数: 0

Abstract

Wheat root systems undergo dynamic and adaptive changes to mitigate the adverse effects through elaborate regulatory mechanisms under drought stress. Elucidating and utilizing these mechanisms is highly important for breeding drought resistant wheat varieties. Here, we identify histone deacetylase TaHDA8, as a critical component in regulating wheat root elongation and drought resistance. Under drought stress, TaHDA8 can be finely tuned to alleviate its inhibition of root elongation, thereby adapting to water deficit. Interestingly, the reduction in TaHDA8 protein levels restores the DNA-binding ability of TaAREB3, a positive regulator of root elongation and drought resistance, which depends on the retention of acetylation at K248 and K281 residues. The restored DNA-binding ability of TaAREB3 activates the expression of TaKOR1, thus promoting root elongation by regulating cell proliferation in the root meristem. Further studies reveal that natural variations in the TaKOR1 promoter determine the differences in TaAREB3 binding, and wheat germplasm with TaHDA8-TaAREB3-TaKOR1 regulatory module has been widely selected. Overall, this study reveals how lysine deacetylases regulate drought-responsive root development via non-histone deacetylation, providing genetic resources for improving root architecture and breeding drought-resistant wheat.

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抑制tahda8介导的TaAREB3赖氨酸去乙酰化是小麦根系发育的干旱适应机制

小麦根系在干旱胁迫下通过复杂的调控机制发生动态和适应性变化，以减轻不利影响。阐明和利用这些机制对选育抗旱小麦品种具有重要意义。在这里，我们发现组蛋白去乙酰化酶TaHDA8是调节小麦根系伸长和抗旱性的关键成分。在干旱胁迫下，TaHDA8可以通过微调来减轻对根系伸长的抑制，从而适应水分亏缺。有趣的是，tada8蛋白水平的降低恢复了TaAREB3的dna结合能力，TaAREB3是根伸长和抗旱性的正调节因子，这取决于K248和K281残基乙酰化的保留。TaAREB3恢复的dna结合能力激活TaKOR1的表达，从而通过调节根分生组织细胞增殖促进根伸长。进一步的研究表明，TaKOR1启动子的自然变异决定了TaAREB3结合的差异，具有TaHDA8-TaAREB3-TaKOR1调控模块的小麦种质已被广泛选择。综上所述，本研究揭示了赖氨酸去乙酰化酶如何通过非组蛋白去乙酰化调控干旱响应性根系发育，为改善根系构型和选育抗旱小麦提供遗传资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Plant 植物科学-生化与分子生物学

CiteScore

37.60

自引率

2.20%

发文量

1784

审稿时长

1 months

期刊介绍： Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.