Use of Whole-genome Sequencing in a Tuberculosis Outbreak among Young Immigrants in a Japanese Language School, 2024.

IF 1.5 Q4 INFECTIOUS DISEASES
International Journal of Mycobacteriology Pub Date : 2025-04-01 Epub Date: 2025-06-20 DOI:10.4103/ijmy.ijmy_46_25
Yoshika Shimokawa, Mizuki Inoue, Risa Sonoda, Chie Hanatate, Mayumi Watanabe, Kaori Tsuzaki, Masaki Ota, Susumu Hirao, Yoshiro Murase, Satoshi Mitarai, Sayoko Koga
{"title":"Use of Whole-genome Sequencing in a Tuberculosis Outbreak among Young Immigrants in a Japanese Language School, 2024.","authors":"Yoshika Shimokawa, Mizuki Inoue, Risa Sonoda, Chie Hanatate, Mayumi Watanabe, Kaori Tsuzaki, Masaki Ota, Susumu Hirao, Yoshiro Murase, Satoshi Mitarai, Sayoko Koga","doi":"10.4103/ijmy.ijmy_46_25","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In early April 2024, three Nepalese students (Pt1-Pt3) in the morning classes of a Japanese language school in Western Japan were diagnosed with sputum smear-positive pulmonary tuberculosis (TB). The smear status of Pt1 and Pt3 was 3+, whereas Pt2 was scanty positive. This study aims at describing cases with active TB as well as latent TB infection (LTBI) in this outbreak.</p><p><strong>Methods: </strong>The outbreak cases are epidemiologically described in terms of time, place, and person.</p><p><strong>Results: </strong>An intensive contact investigation using chest X-rays found 13 cases with active TB as of January 2025. Ten cases, including Pt1 and Pt2, were found in Classroom A, whereas one case each was found in the morning classes of Classrooms B (Pt3) and C and in Classroom D (afternoon class). In addition, 10 other cases of LTBI were found in Classroom A. Whole-genome sequencing (WGS) revealed that Pt3 and another case in Classroom A had genetically distinct Mycobacterium tuberculosis strains, with 1072 and 466 single nucleotide variants, respectively, indicating they were unrelated to the outbreak. On the other hand, the case of Classroom C shared the outbreak strain, and the person had frequently visited the apartment of Pt1 and Pt2. The case of Classroom D did not have close contact with those of Classroom A and was eventually excluded from the outbreak cases.</p><p><strong>Conclusion: </strong>WGS is useful in distinguishing outbreak-related cases from coincidentally found ones in TB outbreak investigations.</p>","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 2","pages":"164-169"},"PeriodicalIF":1.5000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Mycobacteriology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ijmy.ijmy_46_25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/20 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Background: In early April 2024, three Nepalese students (Pt1-Pt3) in the morning classes of a Japanese language school in Western Japan were diagnosed with sputum smear-positive pulmonary tuberculosis (TB). The smear status of Pt1 and Pt3 was 3+, whereas Pt2 was scanty positive. This study aims at describing cases with active TB as well as latent TB infection (LTBI) in this outbreak.

Methods: The outbreak cases are epidemiologically described in terms of time, place, and person.

Results: An intensive contact investigation using chest X-rays found 13 cases with active TB as of January 2025. Ten cases, including Pt1 and Pt2, were found in Classroom A, whereas one case each was found in the morning classes of Classrooms B (Pt3) and C and in Classroom D (afternoon class). In addition, 10 other cases of LTBI were found in Classroom A. Whole-genome sequencing (WGS) revealed that Pt3 and another case in Classroom A had genetically distinct Mycobacterium tuberculosis strains, with 1072 and 466 single nucleotide variants, respectively, indicating they were unrelated to the outbreak. On the other hand, the case of Classroom C shared the outbreak strain, and the person had frequently visited the apartment of Pt1 and Pt2. The case of Classroom D did not have close contact with those of Classroom A and was eventually excluded from the outbreak cases.

Conclusion: WGS is useful in distinguishing outbreak-related cases from coincidentally found ones in TB outbreak investigations.

全基因组测序在日本语言学校年轻移民结核病爆发中的应用,2024。
背景:2024年4月初,三名尼泊尔学生(Pt1-Pt3)在日本西部一所日语学校的早班被诊断为痰涂阳肺结核(TB)。Pt1和Pt3的涂片状态为3+,而Pt2为少量阳性。本研究旨在描述此次暴发中活动性结核和潜伏性结核感染(LTBI)的病例。方法:对暴发病例进行时间、地点和人员的流行病学描述。结果:截至2025年1月,使用胸部x光进行密集接触调查发现13例活动性结核病。A班共发现Pt1、Pt2 10例,B班(Pt3)、C班(上午班)和D班(下午班)各1例。此外,在A教室还发现了另外10例LTBI病例。全基因组测序(WGS)显示,Pt3和A教室的另一例病例具有遗传上不同的结核分枝杆菌菌株,分别有1072和466个单核苷酸变异,表明它们与此次暴发无关。另一方面,C教室的病例共享爆发菌株,并且该人经常访问Pt1和Pt2的公寓。D教室的病例与A教室的病例没有密切接触,最终被排除在暴发病例之外。结论:WGS在结核暴发调查中可用于区分与暴发相关的病例和偶然发现的病例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.20
自引率
25.00%
发文量
62
审稿时长
7 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信